Transcription discussion

Message Strand Traits

Non-Template

Coding

Sense

(+)

Non-Message Strand

Template

Non-coding

Antisense

(-)

Replication Bubble Size

Increases over time

Transcription Bubble Size

Stays the same

Replication Synthesis Direction

Bidirectional

Transcription Synthesis Direction

Unidirectional

Alpha

Determine the DNA to be transcribed

Beta

Catalyze polymerization

Beta Prime

Bind and Open DNA Template

Sigma

Recognize Promotor for Synthesis Initiation

TTGACA Consensus Location (prokaryote

-35

TATAAT Consensus location (pro-euk?)

-10, -25

RNA poly III, Type 2 Promotor: Cis elements

Box A

Box B

RNA poly III, Type 2 Promotor: Trans-acting elements

TFIII-B (Contains TATA Binding Protein)

TFII-C (2 copies)

RNA poly III, Type 2 Promotor: Product

pre-tRNA

RNA poly III, Type 2 Promotor Structure

RNA Poly I: Product

18S

28S

5.8S rRNA

RNA Poly II: Product

pre-mRNA,

miRNA

DNA Poly III T1: Product

5S rRNA

DNA Poly III T3: Product

snRNA