GASP Refresher and Isolation of GASP Mutants

These flashcards cover key concepts from the lecture notes on GASP mutants and their isolation.

GASP

Growth advantage in stationary phase.

Subculture

To transfer a portion of a microbial culture to a fresh growth medium.

Vibrio natriegens

Gram-negative marine heterotroph known for its fast replication and versatile metabolism.

TCBS agar

Thiosulfate–citrate–bile salts–sucrose agar, a selective medium for isolating Vibrio species.

Whole-genome sequencing

A method used to determine the complete DNA sequence of an organism's genome.

Mutant phenotype

The observable characteristics of an organism resulting from mutations.

Cryopreservation

A process to preserve cells or tissues by cooling them to sub-zero temperatures.

Essential genes

Genes that are necessary for the survival of an organism in a given environment.

Antibiotic plate

A growth medium that contains antibiotics to select for resistant bacterial species.

Mutation

A change in the DNA sequence that can lead to variations in traits.

Highly selective culture medium

A type of medium designed to permit the growth of specific organisms while inhibiting others.