Antibody Labelling and General Uses
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10 Terms
Why are antibodies often labelled?
To detect antigen binding
What must a label not interfere with?
Antibody-antigen binding
Name 2 enzyme labels used on antibodies
HRP and alkaline phosphatase (AP)
Name 2 fluorochromes used to label antibodies
FITC and PE
What does detection of a labelled antibody confirm?
Presence of the antigen
What is a secondary antibody?
An antibody that binds to the primary antibody
Why are secondary antibodies used?
To increase sensitivity
How does a secondary antibody increase signal strength?
Multiple secondary antibodies bind one primary antibody
Why are antibodies ideal in research tools?
Due to their specificity
Name one research application of antibodies
Affinity chromatography or immunoflurorescence
Note 
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