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In redox reactions, the loss of electrons from one substance is called ________, the addition of electrons is called _________
oxidation, reduction
LEO goes GER
Loses Electrons is Oxidation
Gains Electrons is Reduction
Explain redox in cellular respiration
High-energy electrons are taken away from glucose and given to oxygen (glucose is oxidized)
As electrons are take away from glucose it forms CO2, as they’re given to oxygen it forms water
What are NAD+ and FAD known as?
Oxidizing agents, coenzymes
What are the two major phases of Glycolosis?
Energy investment phase, energy harvesting phase
What is the energy investment phase?
2 ATP are used to split the glucose into two G3P molecules
What is the energy harvesting phase?
Each G3P is oxidied into pyruvate
Explain redox in photosnythesis
Electrons are taken from water (oxidation) and given to carbon dioxide (reduction)
The oxidation of water forms oxygen
The reduction of carbon dioxide forms a carbohydrate
What do the light reactions do in photosnythesis?
Split water, release O2, produce ATP, and form NADPH
What does the Calvin Cycle do in photosnythesis?
forms sugar from CO2, using ATP and NADPH
what is the electron carrier molecule used in photosnythesis?
NADP+
Why does PS II split water
The electrons from the water are used to replace the electrons lost by the chlorophyll molecuels
What are the numbers for the PhotoSystems
PII: 680
PI: 700
What are the steps in Light Reactions for Photosnythesis
PS II
ETC
PS I
NADP + Reductase
ATP Synthase
What is the final electron acceptor in Photosynthesis?
NADP+
What is the starting molecule in the Calvin Cycle and how many carbons?
5-carbon molucule, RuBP
What are the three phases of the Calvin Cycle
Carbon Fixation
Reduction
Regeneration
How many Calvin cycles are needed for one G3P?
three
What molecule is the primary energy currency of the cell?
ATP
What is the high-energy bond in ATP called?
Phosphate
What is the term for ATP breakdown?
Hydrolysis
What molecule is formed when ATP loses one phosphate?
ADP
What molecule is formed when ATP loses two phosphates?
AMP
What is the term for energy transfer in cells?
Coupling
What is the term for enzyme shape change?
Induced
What is the term for an enzyme helper?
Cofactor
What is the term for enzyme-substrate complex?
Intermediate
What molecule enters the Krebs cycle?
Acetyl-CoA
What is the waste product of aerobic respiration?
Water
What is the electron carrier in photosnythesis?
NADPH
What is the enzyme that fixes carbon?
Rubisco
What process uses proton gradient to make ATP?
Chemiosmosis
What is the term for electron flow in photosnythesis?
Z-scheme
What process occurs without oxygen?
Fermentation
What is the process of yeast fermentation?
Ethanol
What is the product of muscle fermentation?
Lactate
What enzyme controls glycolosis?
PFK
What is the term for enzyme regulation by ATP?
Allosteric
What is the term for fat breakdown and protein breakdown?
Lipolysis, Proteolysis
What do enzymes do in terms of energy?
Reduces activation energy
What are the ways in which enzymes can speed up reactions?
Orient substrates, can strain bonds, can create a microenvironment, can form temp bonds
Where is Glycolysis, Pyruvate Oxidation/Krebs Cycle, Oxidative Phosphorylation located?
Cytosol, Mitochondrial Matrix, Mitochondrial Cristae
Does Glycolosis require oxygen?
No
What does each step of Cellular Respiration produce?
Glycolysis: 2 NADH, 2 Pyruvate, 2 ATPS
Krebs: 2 CO2, 3 NADH, 1 FADH2, 1 ATP (X2)
Oxidative Phosphorylation: 34 ATP
What does Pyruvate into, and what starts the Krebs Cycle?
Acetyl CoA (1 for each Pyruvate)
What does Oxidative Phosphorylatoin start with?
NADH, FADH2 (electrons)
Does the ETC make make energy?
No, it makes protons which are used to make energy in chemiosmosis
Where do the stages of Photosynthesis take place?
Light Reactions: Thylakoid Membrane
Calvin Cycle: Stroma (Cytoplasm)
What are the product of Photosnythesis?
Light Reaction: ATP, NADPH
What does Cyclic Electron Flow do?
Only uses PS 1, synthesizes ATP only
What does the Calvin Cycle start with?
Co2, ATP, NADPH
What does CO2 bind to in the Calvin Cycle?
RuBP
What attaches RuBP and Co2?
Rubisco
Calvin Cycle pathway
RuBP (5 carbon)
Co2 (1 carbon)
They break into 2 3 carbon molecules
Energy comes in
Creates G3P, and is recycled
FRQ 1: plants
B) What’s speciial about C4 plants?
C) CAM plants?
D) Why would C3 be the highest?
B) They use PEP carboxylase to fix CO2 in mesophyll cells, forming a 4-carbon compound that is transported to bundle sheath cells. PEP carboxylase does not bind O2.
C) C3 plants lack the other storing CO2 methods CAM & C4 have
FRQ 2
A) why would oxygen consumption increase with an uncoupling protein despite low ATP production?
B) How will NADH and FADH2 levels change?
C) What is an advantage of uncoupling proteins?
A) It dissipates the proton gradient, but the ETC continues running, increasing oxygen consumption
B) They will be lower because the ETC will run faster. they will be OXIDIZED more rapidly to donate electrons, regeneterating NAD+ and FAD.
C) heat
FRQ 3
A) Describe the role of oxygen in cellular respiration
C) What is the relationship between glucose and ATP concentration?
A) Oxygen is the final electron acceptor. As oxygen levels increase, ATP production increases, showing oxygen is required for ETC.
C) Glycolysis alone doesn’t make much ATP
FRQ 4
A) What happens inside the thylakoid space of the chloroplast?
A) Bright light excites electrons in P.S. II, causing water to be split, releasing protons. Electrons move through the ETC, while more protons are pumped, increasing the proton gradient.
FRQ 6
A) Design an experiment to test light color
B) Purple leaf plant and optium and suboptium
A) Grow plants under different light colors, measure biomass. IV: light wavelength, DP: Plant biomass accumulation
B) Purple leaves contain pigments that absorb red and blue light, so those wavelengths are less available to chlorophyll. Wavelengths absorbed less (green/yellow) penetrate better.
FRQ 7
B) What are the stages of metabolism responsible for CO2 release in both aerobic and anaerobic conditions
C) Why must fermentation occur without oxygen present and how does it allow gylcolysis to occur?
D) How will intracellular ATP levels impact PFK
B) Anaerobic: fermentation where pyruvate is converted to ethanol
Aerobic: Krebs Cycle/Pyruvate oxidation
C) Fermentation regenerates NAD+ from NADH when O2 is absent. This allows glycolysis to continue producing ATP.
D) It will INCREASE PFK activity. Because ATP is an inhibitor of PFK, low ATP reduces inhibition, causing positive feedback regulation
FRQ 8
A) How can ATP concentration decrease
B) If an Exergonic reaction occurs, what reaction must occur with it?
A) when ATP is hydrolyzed faster than its regeneration, like during high cellular activity
B) Endergonic, the energy rleeased is used to drive processes like active transport
FRQ 9
B) Explain why a Catabolic reaction releases energy?
C) Why would anabolic pathways be used in protein synthesis?
B) The breakdown of complex molecules forms more stable products with lower free energy. Cellular respiration breaks down glucose.
C) Builds complex protein molecules from individual amino acids. Energy is required to form peptide bonds.
FRQ 10
Too high temp….
Denature enzymes