ASCP MLT BOC Calculations and Reference Ranges

% Iron saturation calculation

(Total Iron/TIBC) X 100

TIBC formula

UIBC + serum iron

UIBC formula

TIBC - serum iron

Unconjugated/indirect bilirubin calculation

Total bilirubin - direct bilirubin

Friedwald equation/LDL calculation

LDL = CHOL - (HDL + VLDL)

VLDL = Triglycerides/5

non-HDL calculation

Total cholesterol - HDL cholesterol

A/G ratio

albumin/(total protein-albumin)

Creatinine clearance calculation

UV/P x 1.73/A

Beer's law

A= Ebc

A is absorbance

E is absorptibity

b is the light path in centimeters

c is concentration

Corrected WBC counts when > 10 nRBCs present

(automated wbc count x 100) / (NRBCs per 100 wbcs) + 100

Manual hemocytometer counts

(Number Cells Counted x Dilution Factor)/(Total area counted x Depth (0.1))

MCV formula

(Hct x 10)/RBC

Absolute cell counts given the relative values (e.g., WBCs, reticulocytes)

Total white blood cell count x % of cell type

MCH formula

(Hgb x 10)/RBC

MCHC formula

(Hgb x 100) / Hct

Sodium

136 - 145 mmol/L

Potassium

3.5 - 5.1 mmol/L

Chloride

98 - 107 mmol/L

Total CO2

22 - 33 mmol/L

Creatinine

0.8-1.2 mg/dL

71 - 106 µmol/L

Blood urea nitrogen (BUN)

6 - 20 mg/dL

2.1 - 7.1 mmol/L

Glucose (fasting)

74 - 100 mg/dL

4.1 - 5.6 mmol/L

Hemoglobin A1C

< 5.7%

< 39 mmol/mol

Haptoglobin

30 - 200 mg/dL

0.3 - 2.0 g/L

pH

7.35 - 7.45

pCO2

35 - 44 mmHg

4.7 - 5.9 kPa

pO2

> 80 mmHg

> 10.6 kPa

O2 saturation

> 95%

HCO3 - (bicarbonate)

23 - 29 mmol/L

RBC

4.00 - 6.00 x 10^6/μL

4.00 - 6.00 x 10^12/L

HGB

12.0 - 18.0 g/dL

120 - 180 g/L

HCT

35% - 50%

0.35 - 0.50 L/L

MCV

76 - 100 fL

MCH

26 - 34 pg

MCHC

32 - 36 g/dL

320 - 360 g/L

RDW

11.5 - 14.5%

0.115 - 0.145

Reticulocytes (absolute)

20 - 115 x 10^3 /μL

20 - 115 x 10^9 /L

Reticulocytes (relative)

0.5 - 2.5%

0.005 - 0.025

nRBCs

0 nRBC/100 WBC

Platelets

150 - 450 x 10^3/μL

150 - 450 x 10^9 /L

WBC (Total)

3.6 - 10.6 x 10^3 /μL

3.6 - 10.6 x 109/L

Neutrophils (absolute)

1.7 - 7.5 x 10^3/μL

1.7 - 7.5 x 10^9 /L

Neutrophils (relative)

50 - 70%

0.50 - 0.70

Lymphocytes (absolute)

1.0 - 3.2 x 10^3/μL

1.0 - 3.2 x 10^9 /L

Lymphocytes (relative)

18 - 42%

0.18 - 0.42

Monocytes (absolute)

0.1 - 1.3 x 10^3 /μL

0.1 - 1.3 x 10^9 /L

Monocytes (relative)

2 - 11%

0.02 - 0.11

Eosinophils (absolute)

0 - 0.3 x 10^3 /μL

0 - 0.3 x 10^9 /L

Eosinophils (relative)

1 - 3%

0.01 - 0.03

Basophils (absolute)

0 - 0.2 x 10^3 /μL

0 - 0.2 x 10^9 /L

Basophils (relative)

0 - 2%

0.00 - 0.02

CSF WBC/RBC

0 - 5/μL

0 - 5 x 10^6 /L

CSF Glucose

50 - 80 mg/dL

2.8 - 4.4 mmol/L

CSF Protein

15 - 45 mg/dL

150 - 450 mg/L

Seminal Fluid Volume

2-5 mL

Seminal Fluid sperm concentration

> 20 x 10^6/mL

> 20 x 10^9 /L

Urine Specific gravity

1.003 - 1.035

Urine pH

4.5 - 8.0

Urine protein

< 10 mg/dL, trace, or

negative

Urine bilirubin

negative

Urine blood

negative

Urine glucose

≤ 15 mg/dL or negative

≤ 0.8 mmol/L or negative

Urine nitrite

negative

Urine leukocyte esterase

negative

Urine urobilinogen

< 1.0 EU

< 17.0 μmol/L

Urine ketones

< 5 mg/dL or negative

< 0.5 mmol/L or negative

Urine microscopic RBC

0 - 3/HPF

Urine microscopic WBC

0 - 8/HPF

Urine microscopic casts

0 - 2 hyaline/LPF

Urine microscopic epithelial cells

0 - 5/HPF