DNA extraction
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6 Terms
what is the purpose of the detergent in the DNA extraction process?
the detergent breaks down cell surface membranes and nuclear membranes, releasing the DNA
why is salt added in the DNA extraction process?
the salt makes the DNA clump together
why is the mixture in DNA extraction heated in a water bath at 60°C?
heating at high temperatures breaks down enzymes that could damage the DNA and helps dissolve membranes
why is the mixture in DNA extraction filtered after heating?
filtering removes large cell debris and insoluble material, leaving the DNA in solution
why is ice cold ethanol added to the filtrate?
DNA is insoluble in ethanol and so forms a precipitate
how can the extracted DNA be collected?
the DNA forms a white precipitate that can be wound around a glass stirring rod and lifted out
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