DNA extraction

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6 Terms

1
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what is the purpose of the detergent in the DNA extraction process?

the detergent breaks down cell surface membranes and nuclear membranes, releasing the DNA

2
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why is salt added in the DNA extraction process?

the salt makes the DNA clump together

3
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why is the mixture in DNA extraction heated in a water bath at 60°C?

heating at high temperatures breaks down enzymes that could damage the DNA and helps dissolve membranes

4
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why is the mixture in DNA extraction filtered after heating?

filtering removes large cell debris and insoluble material, leaving the DNA in solution

5
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why is ice cold ethanol added to the filtrate?

DNA is insoluble in ethanol and so forms a precipitate

6
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how can the extracted DNA be collected?

the DNA forms a white precipitate that can be wound around a glass stirring rod and lifted out