PCR Crash Course Part II
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6 Terms
If PCR can’t be used to detect RNA, and coronaviruses are RNA viruses; there is no DNA and PCR does not replicate RNA, how can we detefct SARS-Cov-2?
use qPCR to detect DNA
we know of a method of creating RNA from DNA
What is RT-qPCR?
is reverse transcriptase-qPCR
Reverse transcription is an enzyme-mediated process by which complementary DNA (cDNA) molecules are created from RNA templates.
After reverse transcription is completed, qPCR can continue as explained previously using cDNA as the template.
Performing RT-qPCR
sample (template)
Luna Enzyme (reverse transcriptase)
Primer Set
Probe Set
Luna Buffer (DNA polymerase)
Performing qPCR-Sample Concentration
Great sample concentration=?
Earlier detection of amplification
LoD (LoD)
The lowest concentration of target DNA that can be detected during amplification
In Lab Experiment
Synthetic Human DNA (P1)
Synthetic SARS-COV-2 RNA (N1)
Note 
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