(f) the effects of inhibitors on the rate of enzyme controlled reactions

What are inhibitors used for?

Enzymes are inactivated by these

What are inhibitors used for?

Molecules that prevent enzymes carrying out their normal functions

What is a competitive inhibiton

A molecule or part of a molecule that has similar shape to the substrate of enzyme binds to active site

Blocks the substrate from binding

Enzyme is inhibted

Most competive inhibitors bind temporarily but

Some dont like aspirin

Why are competive inhibitors competive

Substrate and inhibitor molecules compete to bound to the active site.

This reduces the number of substrates binding to a.s in given time

What does the degree of inhibitions depend on

Concentration of enzyme + inhibitors + substrate molecules

What is an example of competive inhibitors

Statins

Help reduce cholesterol in blood

What affect do competive inhibitors have on rate of reaction

It decreases but not the v max of enzyme because substrate molecule can be increased to still reach the v max.

What do non competive inhibition

Bonds to alllosteric part of the enzyme

Changes shape fo t. Structure of enzyme

Active site changes shape

No longer complementary

Enzyme cant bind- can perform its function

Will increasing the substrate or enzyme overcome the effect of the inhibitor

No

What does an increase in concentration of inhibitor do

Decrease rate of reaction (more active sites become unavailable)

Binding of inhibitor is either

Reversible or irreversible

What is an irreversible binding inhibitor

Can be toxic

Can’t be removed from part of enzyme it’s attached to

What are some examples non reversible inhibitors

Proto pump inhibitors used to treat lng tem indigestion

Reduces production of excessive acid (inhibits enzyme that secretes hydrogen ions)

What is end product inhibition and what is the use of it

Occurs when product of enzyme reaction inhibits the enzyme itself

Negative feedback control system for reaction

Excess products are not made

Resoruces are not wasted

What is end product inhibition an example of

Non competitive

Reversible reaction