2.3 More Microscopy

Name some features of a light microscope

• Easily transportable

• Easy to use

• Relatively cheap to use

Can you see living or dead organisms with a light microscope?

Both

What is the maximum magnification of light microscopes?

X2000

What is the resolution of light microscopes?

0.2µm

What does TEM stand for?

Transmission Electron Microscope

What does SEM stand for?

Scanning Electron Microscope

What is the maximum magnification of a TEM?

X500 000

What is the maximum magnification of a SEM?

X500 000

What is the resolving power of a TEM?

0.5nm

What is the resolving power of a SEM?

3-10nm

Is the image produced by a TEM in colour?

No- it’s black and white

Is the image produced by a SEM in colour?

No- it’s black and white

Is the image produced by a TEM 2D or 3D?

2D

Is the image produced by a SEM 2D or 3D?

3D

Does a TEM show internal or external structures?

Internal structures

Does a SEM show internal or external structures?

External structures

Do the specimens used in TEMs have to be alive or dead?

Dead

Do the specimens used in SEMs have to be alive or dead?

Dead

What are some advantages of laser scanning confocal microscopes?

• You can observe tissue or small living organisms

• Used on thick or 3D specimens

• High resolution

What are some disadvantages of laser scanning confocal microscopes?

• Slow to use

• Can cause photodamage to cells