Lab #10 - Hydrogen Sulfide, Indole, Motility, Methyl Red, Voges-Proskauer, Citrate, Urea Tests

These flashcards cover key concepts and definitions relevant to the hydrogen sulfide, indole, motility, methyl red, Voges-Proskauer, citrate, and urea tests conducted in Lab #10.

Hydrogen Sulfide Test

A test determining whether a bacterium can produce hydrogen sulfide gas, creating a black precipitate in the medium.

Indole Test

A test to detect indole production from tryptophan in bacteria, which is indicated by a red color after adding Kovac’s reagent.

Motility Test

A test that assesses whether bacteria can move in a semisolid medium, indicated by growth throughout the tube.

Methyl Red Test

A test that indicates mixed acid fermentation, where a red color indicates a pH of 4 or less after adding methyl red reagent.

Voges-Proskauer Test

A test that detects butanediol fermentation by checking for acetoin, indicated by a red color after adding Barritt’s reagents.

Citrate Test

A test determining if a bacterium can utilize citrate as a carbon source, indicated by a color change from green to blue in the medium.

Urea Test

A test indicating whether bacteria produce urease to use urea; a color change to dark pink indicates a positive result.

Facultative Anaerobes

Bacteria capable of living in both oxygen-rich and oxygen-poor environments, such as those in the Enterobacteriaceae family.

SIM Test

A test that combines hydrogen sulfide production, indole formation, and motility assessment in a single medium.

IMViC Tests

A series of four tests (Indole, Methyl Red, Voges-Proskauer, Citrate) used to differentiate members of the Enterobacteriaceae family.