AMNIOTIC FLUID - LEC

contaminated with maternal blood, tissue fluid and cells

Discard first 3-5ml

Hydramnios

Abnormal increase of the fluid volume

Decreased fetal swallowing

Congenital fetal malformations

Oligohydramnios

Abnormal decrease of the fluid volume

Congenital fetal malformations

Point of reference: COLOR

Amniotic Fluid: Pale yellow
Maternal Urine: Pale yellow

Point of reference: Creatinine

Amniotic Fluid: 3.5mg/dl
Maternal Urine:10mg/dl

Point of reference: Urea

Amniotic Fluid: 30mg/dl
Maternal Urine: 300mg/dl

Point of reference: Glucose

Amniotic Fluid: Present
Maternal Urine: Absent

Point of reference: Protein

Amniotic Fluid: Present
Maternal Urine: Absent

Appearance

Normal: Colorless to pale yellow

Abnormal: Yellow, Green, Red-brown, Amber, Pink-red

Clinical Correlation: Erythroblastosis

Fetal hypoxi

Fetal death

(+) bilirubin

Blood contamination

Volume

Normal: 1,000-1,500mL at term (36 weeks)

Abnormal: Hydramnios

Oligo-hydram-nios

Clinical Correlation: Abnormal increase, Abnormal decrease

Amniostat FLM

• Immunologic agglutination test for phosphatidyl glycerol

• Provides rapid method for assessment of lung maturity

Lamellar Bodies Count

The count correlates with the amount of

phospholipids present in fetal lung

Kleihauer-betke Test

• If maternal blood is present = colorless when stained

• If fetal blood is present = purple – pink when stained

Lecithin

• Primary surface fluid

• Produced at relatively low & constant rate until 35th week of gestation

Sphingomyelin

Produced at constant rate after about 26th week of gestation

Phosphatidyl glycerol

Essential fluid for adequate lung maturity

Spectrophotometric measurement of light

Reflectance refractometry

Light-Emitted Diode (LED)

provide the specific wavelength needed for each test pad color reaction