AMNIOTIC FLUID

35mL amniotic fluid is derived from Maternal Blood/plasma

1st Trimester: ______mL of amniotic fluid is derived from _____

Fetal urine becomes the major contributor of amniotic fluid volume

What happens to amniotic fluid AFTER 1st TRIMESTER

800 - 1200 mL (average: 1 liter)

Normal amniotic volume

Max of 30 mL, first 2 or 3 mL discarded _{^{[contaminated with maternal blood, tissue fluid, and cells]}}

Proper collection of AMNIOTIC FLUID

2nd Trimester

AMNIOCENTSIS:

• Assess GENETIC DEFECTS (Down Syndrome)

3rd trimester

AMNIOCENTSIS:

• Assess Fetal Lung Maturity (FLM) and Hemolytic Disease fo Newborn (HDN)

14th week of gestation

When is Amniocentesis SAFE TO PERFORM

• 16th week gestation

• _{^{15th to 18th week gestation}}

• _{^{20th to 42 week gestation}}

AMNIOCENTESIS: Week Gestation

• for CHROMOSOMAL ANALYSIS

• _{^{16th week gestation}}

• 15th to 18th week gestation

• _{^{20th to 42 week gestation}}

AMNIOCENTESIS: Week Gestation

• for Triple marker screening, Genetic Assessment

• _{^{16th week gestation}}

• _{^{15th to 18th week gestation}}

• 20th to 42 week gestation

AMNIOCENTESIS: Week Gestation

• for FLM, Fetal Distress, HDN

Fern test

TEST:

• Used to evaluate Premature Rupture of Membrane

Bilirubin Analysis

TEST:

• For evaluation of Hemolytic Disease of Newborn

• Bilirubin = 450 nm

• Oxyhemoglobin = 410 nm

• Bilirubin = __ nm

• Oxyhemoglobin = __ nm

Unacceptable

• removed by extracting choloroform

For BILIRUBIN ANALSYSIS, specimen contaminated with blood is _____

A450

katong number sa LILEY GRAPH _{^{—for amniotic bilirubin conc.}}

• Alpha-fetoprotein (AFP) —screening

• Acetylcholinesterase (Ache) —confirmatory

TESTS FOR NEURAL TUBE DEFECTS

Respiratory Distress Syndrome (RDS)

Most common complication of early delivery

• Lecithin-Sphingomyelin (L/S) ratio

• Amniostat-Film

• Foam Stability Index

• Optical Density

• Lamellar Body Count

TESTS FOR FETAL LUNG MATURITY

Creatinine Level

Test for determining fetal age

35th weeks gestation

Phosphatidyglycerol (PG) can be detected after _____

• Lecithin = 35th weeks gestation _{^{[same with PG]}}

• Spingomyelin = 26th weeks gestation

WEEKS GESTATION:

• Lecithin =

• Spingomyelin =