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What are the steps of signal transduction
Cell receives signal through receptor, transduces and amplifies signal, elicits response
What are common second messengers in plants
cAMP, cGMP, and Ca2+ (calcium ions)
What is a phosphorylation cascade
Addition of phosphate group to a protein catalyzes addition of phosphate to subsequent protein in pathway until final response
What are kinases
Enzymes that add phosphate groups to specific proteins
What are phosphatases
Enzymes that remove phosphate groups from proteins
What is etiolation
Morphological adaptations when flowering plant is grown in dark - includes long thin stems, small leaves, lack of chlorophyll (chlorosis)
Why does etiolation occur
Plant's attempt to reach light source by focusing energy on stem elongation and expansion
What is de-etiolation
Process when plant reaches light source - stem elongation slows, leaves expand, roots grow, chlorophyll production increases (greening)
What responds to light during de-etiolation
Phytochromes that respond to red and far-red light imbalances
Who discovered auxin
Charles Darwin (instrumental in discovery of phototropism and auxin)
What is auxin involved in
Phototropism, apical dominance, and shoot elongation
What is the main form of auxin
Indoleacetic acid (IAA) - a small polar molecule with two rings
Where is auxin produced
In the shoot tip
How is auxin transported
Downwards towards base through polar transport using PIN proteins (efflux transporters)
What is the acid growth hypothesis
As cell wall pH decreases from H+ pumping, expansins are activated, breaking hydrogen bonds and loosening cell wall, leading to cell elongation
What are expansins
Proteins activated by cell wall acidity that break hydrogen bonds and loosen cell wall
How does auxin cause phototropism
Auxins associate at shaded side, H+ is pumped decreasing pH, expansins activated, cells elongate on shaded side pushing stem toward light
What other roles does auxin have
Activating growth-related proteins, controlling branch formation, determining phyllotaxy (leaf arrangement)
What are cytokinins derived from
Adenine
Why are cytokinins named so
Due to their role in promoting cytokinesis
What is the most common naturally occurring cytokinin
Zeatin (first found in Zea mays/corn)
Where are cytokinins produced
Growing tissues like roots, fruits, and embryos
How are cytokinins transported
From roots to shoots through xylem sap
What do cytokinins regulate
Cell division, growth, and apical dominance
What is the auxin to cytokinin ratio important for
Plant growth and cell division
What happens when only cytokinins are present
Cultured plant cells cannot divide
What happens with balanced auxin and cytokinins
Cell division occurs and callus (undifferentiated parenchyma tissue) forms
What happens with higher cytokinin ratio
Cells form shoot tissue
What happens with higher auxin ratio
Cells form roots
How do cytokinins affect apical dominance
Suppress apical dominance and promote axillary bud growth (opposite of auxin and strigolactones)
What role do cytokinins play in aging
Slow plant aging - leaves stay green longer in cytokinin solution
What disease led to discovery of gibberellins
Foolish seedling disease caused by Gibberella fungus in rice
What do gibberellins primarily control
Stem elongation, fruit growth, and seed germination
Can wild-type plants grow taller with gibberellin treatment
No, they have sufficient gibberellin concentration already
What is bolting
Rapid growth of floral stalk during plant's reproductive stage
How do gibberellins affect grapes
Make them grow larger and increase internode spacing for better air circulation
What determines sex in cucurbits
High gibberellins result in male flowers, high ethylene results in female flowers
What is the gibberellin to ABA ratio important for
Determining if seed germination occurs
What is ABA's main function
Slowing down growth
Was ABA correctly named
No, originally thought to promote leaf abscission but doesn't - ethylene does
What is ABA's primary function
Seed dormancy - high ABA levels inhibit germination
What is precocious/premature germination
When ABA levels are too low and seed germinates in subpar environmental conditions
How do desert plants use ABA
High ABA levels prevent precocious germination
water washes ABA out allowing germination in moist environment
What does ABA do during drought
Closes plant stomata to reduce transpiration and prevent wilting
What makes ethylene unique among plant hormones
It is a gas hormone
What is one function of ethylene
Fruit ripening (why putting fruit in paper bag ripens them)
What is the triple response
Plant encountering barrier slows stem elongation, thickens stem, and curves horizontally
What does sudden increase in ethylene associate with
Apoptosis and senescence
What is an abscission layer
Forms at petiole from cells undergoing apoptosis, leading to leaf abscission
What actually controls leaf abscission
Ethylene (not abscisic acid)
How does ethylene relate to auxin in leaf abscission
As auxin level in leaf falls, ethylene becomes more effective and induces senescence
What happens during fruit ripening
Fruit softens, molecules converted to sugars, produces attractive scents and colors
What initiates fruit ripening
Burst of ethylene with sustained positive feedback loop
What inhibits ethylene
Carbon dioxide
What are ein mutants
Ethylene-insensitive mutants that cannot respond to ethylene and grow higher than usual (no triple response)
What are eto mutants
Ethylene-overproducing mutants that produce too much ethylene and grow shorter than usual (too much triple response)
What are ctr mutants
Constitutive triple response mutants that undergo triple response even without ethylene (very short plants)
What are brassinosteroids similar to
Molecularly similar to cholesterol in animals, functionally similar to auxin
What do brassinosteroids do
Stimulate cell elongation and division in young plant components, slow leaf abscission, promote xylem differentiation
What are jasmonates based on
Fatty acids (linolenic acid)
What is the main function of jasmonates
Plant defense (also nectar production, fruit ripening, pollen production, flowering, germination, root growth, tuber formation, mycorrhizal symbioses, tendril formation)
What are PAMPS
Pathogen-associated molecular patterns - special molecules indicating presence of pathogens
What are phytoalexins
Activated to kill pathogens
What are R genes
Resistance genes that activate specific immune responses
What is hypersensitive response
Local, specific immune response
What is systemic acquired resistance (SAR)
General defense response that spreads through entire plant
What is systemin
Initiates SAR by synthesizing jasmonic acid
What is salicylic acid
Component of SAR that spreads through entire plant prepping immune system
What are raphides
Calcium oxalate crystals in idioblasts that cut herbivore's tongue to deter herbivory
Why can humans eat most plants with raphides
Cooking destroys raphides
What do strigolactones do
Stimulate germination, control apical dominance (with auxin), regulate mycorrhizal associations
Where were strigolactones discovered from
Study of Striga plants (parasites of food-producing grains)
What are blue-light photoreceptors
Receptors that detect blue light from the sun
What are cryptochromes
Blue-light photoreceptors that inhibit stem elongation in response to blue light
What is phototropin
Blue-light receptor that controls stomatal opening and phototropism
What are phytochromes
Red (660 nm) and far-red (730 nm) photoreceptors
What are the two forms of phytochromes
Pr and Pfr
What happens when Pr receives red light
Changes to Pfr state
What happens when Pfr receives far-red light
Converts back to Pr
What does Pfr promote
Germination and horizontal growth
What does Pr promote
Inhibits germination and induces vertical growth
What is a free-running period
Plant's natural rhythm of 21-27 hours determined in constant-light environment
What resets the biological clock in plants
Sudden Pfr increase at sunrise (stimulated by red light)
What are short-day plants
Actually long-night plants - flower when night is longer than critical length
What are long-day plants
Actually short-night plants - flower when night is shorter than critical length
What are neutral-day plants
Flower no matter how long the night is
What happens if night is interrupted by red light in short-day plants
Will NOT flower
What happens if night is interrupted by red light in long-day plants
WILL flower
What flash of light matters for flowering
Only the LAST flash of light
What is vernalization
When plant is exposed to cold temperatures to induce flowering
What is positive gravitropism
Growth downwards (in gravity's direction)
What is negative gravitropism
Growth upwards (against gravity)
What are statoliths
Specialized plastids with starch inside that indicate gravity direction by sinking to bottom of cells
What is thigmotropism
Plant growth in response to touch
How do mimosa and venus flytrap respond to touch
Rapidly fold leaves through rapid loss of turgor pressure via potassium ion loss followed by water loss
What is a plant's goal during drought
Reduce transpiration
How do plants respond to drought
Alter physical structure, wilt or shed, close stomata
increase ABA synthesis
How do plants respond to flooding
Adapt to low-oxygen conditions