Microscopy
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4 Terms
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Function of a Stage Micrometer?
Calibrates the eyepiece graticule so that it can be used to make measurements.
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How is an Eyepiece Graticule Calibrated?
- Select the desired magnification and object
- Place stage micrometer on the stage
- Line up scales of micrometer and eyepiece graticule
- Count number of divisions on the graticule equivalent to each division on the stage micrometer
- Calculate length of one division of the eyepiece graticule
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Preparing a Slide
- Stain the sample with appropriate dye (highlights sub-cellular structures)
- Mount the sample on the slide
- Place cover slip carefully on the slide, to prevent air bubbles
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Magnification
size of image / size of object
Note 
Flashcards (64)