Lab Practical 2

5.0(2)
studied byStudied by 85 people
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
Card Sorting

1/79

flashcard set

Earn XP

Description and Tags

(unfortunately) i cannot add any organ structure pictures

Study Analytics
Name
Mastery
Learn
Test
Matching
Spaced

No study sessions yet.

80 Terms

1
New cards
What does ELISA stand for?
Enzyme-Linked Immunosorbent Assay
2
New cards
What does a strongly positive test vs slightly positive ELISA test indicate?
It indicates the amount of antibodies the patient has. The darker green the test result, the more the patient has.
3
New cards
What is an indicator?
A substance that gives a visible sign, usually by a color change, of the presence or absence of a threshold concentration of a chemical.
4
New cards
What are antigens?
Any substance (usually a protein or carbohydrate) that induces the production of antibodies by immune cells. Usually is a substance that is "foreign" to the host.
5
New cards
What are antibodies?
A type of protein called an immunoglobulin found in the blood that is produced by immune cells in response to the presence of a foreign particle (antigen).
6
New cards
What do antigens and antibodies have to do with ELISA?
In an ELISA, the antigen (target macromolecule) is immobilized on a solid surface (microplate) and then complexed with an antibody that is linked to a reporter enzyme. The most crucial element of an ELISA is a highly specific antibody-antigen interaction.
7
New cards
What does a wet spirometer measure?
It measures lung volumes by capturing air in an inverted chamber.
8
New cards
What are the expected values for TV?
500mL for males and 460mL for females.
9
New cards
What are the expected values for RV?
1200mL for males and 900mL for females.
10
New cards
What do restrictive disorders do to the lungs?
They stop the lungs from fully expanding.
11
New cards
What do obstructive disorders do to the lungs?
They impede the ability to exhale.
12
New cards
Examples of obstructive disorders in the lungs:
COPD, bronchitis, and bronchiolitis.
13
New cards
Examples of inspiratory and expiratory issues of restrictive disorders in the lungs:
Inspiratory issues: Decrease in lung elasticity (scaring/fibrosis) or a problem with expansion of the chest wall during inspiration, muscles too weak to inflate the lungs.

Expiratory issues: Weak muscles (accessory), obesity, and pregnancy.
14
New cards
Lung volumes:
Measurements of air movement into and out or the lungs
15
New cards
Lung capacities:
A sum of two or more lung volumes
16
New cards
TV stands for what?
Tidal Volume. It is the volume of air breathed in a normal breath: in and out.
17
New cards
RV stands for what?
Reserve Volume. The amount of air always left in the lungs.

Your lungs retain 1200 ml, unless a lung collapses!
18
New cards
IRV stands for what?
Inspiratory Reserve Volume. The amount of air that can be inhaled beyond the normal inhalation – during quiet breathing.

Up to 3000ml
19
New cards
ERV stands for what?
Expiratory Reserve Volume. It is forced exhalation after normal inhalation.

About 2000ml
20
New cards
Total lung capacity:
The sum of all lung volumes
21
New cards
Vital capacity:
TV+IRV+ERV
22
New cards
Inspiratory capacity:
TV+IRV
23
New cards
Functional residual capacity:
ERV+RV
24
New cards
Forced expiratory vital capacity:
The amount of air expelled in a specific amount of time, after maximum inhalation.
25
New cards
Thyroid cartilage is more commonly know as what?
Adam’s apple
26
New cards
What does arytenoid cartilage do?
It moves the vocal cords.
27
New cards
Swallowing involves what?
Larynx moves upwards (muscle movement)

Epiglottis covers the glottis (opening)

Prevents food and fluid from entering the trachea (windpipe)
28
New cards
Benedicts solution indicates what?
When a monosaccharide (sugar: glucose) is present.
29
New cards
When adding iodine (yellow/orange) to a starch solution you should expect a?
Dark blue/black color change
30
New cards
What color change happens in a positive Benedicts test?
Blue to orange/red
31
New cards
Amylase is an enzyme that breaks down?
Starch
32
New cards
What are enzymes?
Proteins which facilitate all physiology, act on specific molecules, and often their names end in “ase.”
33
New cards
What are the two forms of amylose?
Salivary and Pancreatic
34
New cards
Starch:
A large, polymer of glucose that is water-insoluble and is a storage of glucose.
35
New cards
Glucose:
A small, water-soluble molecule that cannot be stored because it dissolves in water.
36
New cards
Which enzymes were studied in the spit lab? How are they different?
Salivary amylase and pancreatic amylase.

Salivary glands produce salivary amylase into the mouth and initiates the carbohydrate digestion while the pancreas produces pancreatic amylase into the small intestine and completes carbohydrate digestion.
37
New cards
What are the normal test strip levels for pH?
pH should be slightly acidic (usually 6 or 6.5)
38
New cards
What are the normal test strip levels for glucose?
**None**
39
New cards
What are the normal test strip levels for bilirubin?
None
40
New cards
What are the normal test strip levels for protein?
0 to 14 mg/dL.
41
New cards
What are the normal test strip levels for leukocytes?
None
42
New cards
What may be a problem if urine pH levels are not normal?
It may be a sign of kidney stones.

(your pH varies during the day going from more acid in the am to more alkaline in the pm).
43
New cards
What may be a problem if urine glucose levels are not normal?
A positive urinalysis for glucose can indicate diabetes.

If the pancreas isn’t producing enough insulin, glucose will spill over into the urine.
44
New cards
What may be a problem if urine bilirubin levels are not normal?
It can be an early indication of liver disease.

Though, the reason pee is yellow is because of the bilirubin cycle (the recycling of blood cells by the body).
45
New cards
What may be a problem if urine protein levels are not normal?
It means you may have Proteinuria (protein in the urine). It can indicate kidney disease.
46
New cards
What may be a problem if urine leukocyte levels are not normal?
It can indicate an infection in the kidney or bladder, a possible UTI.
47
New cards
What is the problem if there is blood in the urine?
It could be benign conditions such as a urinary tract infection (UTI), kidney stones, kidney infection, medication, menstruation, or strenuous exercise.

Or it could be an early sign of bladder cancer and upper tract urothelial carcinoma.
48
New cards
Why is urine a good, non-intrusive way to investigate the homeostasis (health/pathology) of the body?
Urinalysis is a good way to monitor the functions of the body because it is known what is normal and what is not. 
49
New cards
What is the path of renal blood flow?
Renal artery -> segmental arteries -> interloper arteries -> arcuate arteries -> cortical radiate arteries -> afferent arterioles -> glomerulus -> efferent arterioles -> peritubular capillaries -> cortical radiate veins -> arcuate veins -> interlobar veins -> renal vein.
50
New cards
What is the path of urine formation?
Glomerulus> Bowman’s Capsule> Renal Tubule> Renal Pelvis> Ureter
51
New cards
What do anti*gens* cause your body to *gen*erate?
Antibodies, which are made by our immune systems to fight off invaders.
52
New cards
The antibodies in the ELISA test are where?
In your serum (the liquid portion of your blood that does NOT contain anticoagulant, blood cells or clotting factors). 
53
New cards
ELISA can be used to test for?
Lupus (an autoimmune disease in which the immune system attacks self and results in widespread inflammation and tissue damage, which affects skin, joints, brain, kidneys, blood vessels, lungs)

or

COVID (if you have ever had a false positive may be that you were exposed to the virus and your body mounted an attack without you experiencing symptoms)
54
New cards
What temperature did we incubate the ELISA samples at?
37 degrees Celsius, which is body temperature: 98.6 Fahrenheit.
55
New cards
What is the path of air?
Nose->Nasopharynx->Oropharynx->Epiglottis->Glottis->Larynx->Trachea->Right or left bronchus->Bronchioles->Alveoli
56
New cards
Where does gas exchange occur?
Alveoli
57
New cards
How many alveoli are there?
400 million
58
New cards
Why are lung volumes higher in males?
It is because their lungs are larger.
59
New cards
The nose is where what happens?
Air is filtered and warmed in the nasal conchae
60
New cards
When you get laryngitis what happens?
You cannot talk because the larynx is the “voice box".”
61
New cards
What does the epiglottis do?
It keeps food and drink out of your lungs.
62
New cards
The glottis is what?
The part of the pharynx that contains the larynx.
63
New cards
The diaphragm is what?
The primary muscle of respiration.
64
New cards
COPD is what and includes what?
Chronic Obstructive Pulmonary (lung) Disease (generally harder to breathe out) and includes: asthma, bronchitis and emphysema (happens after years of smoking/vaping).
65
New cards
The throat model from superior to inferior:
(With the huge hyoid bone at the top), under it is Thyroid Cartilage, then Cricoid Cartilage, then the Trachea. At the back is the Arytenoid Cartilages. The vocal chords are the brown leather part in as you look down.
66
New cards
If you were to follow a piece of food from the beginning of the digestive system to the end what would you pass through (in order)?
Mouth> Oropharynx> Esophagus> Stomach> Duodenum> Jejunum>Ileum> Cecum> Ascending Colon> Transverse Colon> Descending Colon> Sigmoid Colon> Rectum> Anus
67
New cards
How do you determine sex in fetal pigs?
Male fetal pigs have a scrotum.
68
New cards
Mechanical digestion includes what?
Your teeth (which masticate your food and the muscular stomach walls, which contract to help with coating your stomach contents with the Chemicals of Digestion).
69
New cards
Chemicals include proteins that are what?
Protein
70
New cards
Enzymes break down what?
Complex molecules like starch into sugars.

(the enzyme responsible for this is Amylase, which is present in your spit and produced by the Pancreas.)
71
New cards
The indicator for starch is what?
Iodine
72
New cards
If you add salivary Amylase (spit) to starch, you would expect what?
Amylase to start to break down the starch into sugar.
73
New cards
You have 2 of what 2 things?
2 Kidneys and 2 Ureters
74
New cards
You only have 1 of what 2 things?
Bladder and Urethra
75
New cards
The functional unit of the kidney is what?
The nephron
76
New cards
Where is Bowman’s Capsule located?
It is a part of the nephron.
77
New cards
Where is the glomerulus located?
Inside Bowman’s Capsule (which is a part of the nephron).
78
New cards
Will you pass the lab practical?
Yes :)
79
New cards
What lines the stomach?
Gastric folds
80
New cards
What connective tissue anchors the small intestine?
Splanchnic mesoderm