Bromination Procedure

step 1

1. Obtain 8 disposable test tubes.

step 2

1. Add 2 mL of DCM to each of the tubes.

step 3

1. Add 0.5 mL of each substrate to its corresponding numbered tube.

step 4

1. Add 0.5 mL of the bromine solution to the 4 remaining tubes.

step 5

1. Place all 8 tubes into an ice-water bath for 5 minutes.

step 6

1. Add one of the bromine tubes to one of the 4 substrate tubes and record how long it takes to react.  Record the time and then repeat step 6 for the remaining substrates.

step 7

1. Once the order has been determined for each set of tubes, all of the tubes can be poured into an Erlenmeyer flask.

step 8

1. Quench any residual bromine by added a few (3-4) drops of cyclohexene to the flask or enough such that the solution is colorless.

step 9

1. Dispose of the solution in the halogenated waste container.