Lab Practical 3 Reagents

0.0(0)
studied byStudied by 0 people
0.0(0)
full-widthCall Kai
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
GameKnowt Play
Card Sorting

1/25

encourage image

There's no tags or description

Looks like no tags are added yet.

Study Analytics
Name
Mastery
Learn
Test
Matching
Spaced

No study sessions yet.

26 Terms

1
New cards

Phenol Red Fermentation

purpose: fermentation/anaerobic respiration

reagents:

  • one sugar (glucose, lactose, sucrose, adonitol, arabinose, dulcitol, sorbitol)

  • Phenol red (pH indicator)

  • Durham tube

2
New cards

Methyl Red Test

purpose: mixed acid glucose fermentation

reagents:

  • sugar (glucose)

  • Methyl red (pH indicator)

3
New cards

Voges-Proskauer Test

purpose: Butanediol ferment acetoin

reagents:

  • sugar (glucose)

  • VP I: alpha-Naphtol

  • VP II: KOH (potassium hydroxide)

4
New cards

Nitrate Reduction Test

purpose: transform nitrates into nitrites; nitrate reductase
NO3 => NO2 => NH4

reagents:

  • medium with nitrates

  • NR-A: alpha-naphthylamine

  • NR-B: sulfanilic acid

  • Zn (zinc)

5
New cards

Catalase test

purpose: catalase; protect themselves from toxic by-products of oxygen metabolism

reagents:
- H2O2 (hydrogen peroxide)

6
New cards

Oxidase Test

purpose: cytochrome C oxidase; aerobic respiration

reagents:

  • chromogenic reducing agent

7
New cards

Starch Hydrolysis

purpose: hydrolysis starch down to sugar

reagents: agar medium with starch and liquid iodine

8
New cards

SIM test (sulfur)

purpose: cysteine desulfurase; anaerobic respiration

reagents:

  • ferric citrate

  • cysteine

9
New cards

SIM test (indole)

purpose: Tryptophanase (indole)

reagents:

  • Tryptone

  • Kovac's reagent

10
New cards

Urease Test

purpose: urease; hydrolysis urea into ammonia

reagents:

medium with urea

phenol red (pH indicator)

11
New cards

Citrate Utilization Test

purpose: citrate lyase and citrate permease

reagents:

  • sodium nitrate (citrate? in lecture)

  • bromothymol blue (pH indicator)

12
New cards

Decarboxylation Test

purpose: decarboxylase ornithine, arginine, and lysine

reagents:

  • ornithine, lysine, or arginine

  • mineral oil

  • bromocresol purple (pH indicator)

13
New cards

(Phenylalanine) Deamination test

purpose: phenylalanine deaminase

reagents: phenylalanine and ferric chloride

14
New cards

Blood Agar Test

purpose: G+ Streptococci; lytic toxins, RBC

reagents: TSA and sheep blood

15
New cards

Mannitol Salt Agar (MSA) test

purpose: mannitol fermentation; G+ Staphylococci

reagents:

  • mannitol

  • NaCl (salt)

  • phenol red (pH indicator)

16
New cards

Bacitracin/Taxo A test

purpose: Streptococcus and other Beta hemolytic species

17
New cards

Optochin/Taxo P test

purpose: Streptococcus and other alpha hemolytic species

18
New cards

Bile Esculin Test

purpose: esculinase; Enterococcus faecalis and Streptococcus

reagents:

  • esculin

  • ferric citrate

  • bile

19
New cards

Hektoen Enteric Agar

purpose: Salmonella and Shigella; G+ enterobacteria; fermentation and H2S production

20
New cards

MacConkey Agar (MAC)

purpose: G- rods, enterobacteria; lactose fermentation

reagents:
~ lactose
~ neutral red (pH indicator?)

  • bile salts

  • crystal violet

21
New cards

6.5% NaCl tolerance test

purpose: Entercoccus faecalis and other Streptococcus

reagents:

  • salts

  • TSB

22
New cards

Coagulase Test

purpose: G+ Staphlococcus; coagulate plasma

Reagents:

  • rabbit plasma

23
New cards

DNAse test

purpose: G+ Staphylococcus; DNAse enzyme

reagents:

  • plate with precipitated DNA

24
New cards

Eosin Methylene Blue Agar (EMB)

purpose: G- enterobacteria; lactose fermentation

reagents:

  • eosin

  • methylene blue

  • lactose

25
New cards

Xylose Lysine Deoxycholate (XLD) agar

purpose: Salmonella, Shigella, and other G- enterobacteria; fermentation and H2S production

reagents:

  • bile acids

  • Xylose (sugar)

  • Lysine (amino acid)

  • lactose and sucrose

  • phenol red (pH indicators)

  • sodium thiosulfate

  • ferric ammonium citrate

26
New cards

Salmonella-Shigella Agar (SS)

purpose: pathogenic enteric bacteria; Salmonella and Shigella; lactose fermentation and H2S production

reagents:

  • bile salts

  • sodium citrate

  • brilliant green

  • lactose

  • neutral red

  • thiosulfate

  • ferric citrate