Unit 4a - Antibody Production; 4b = Dilutions/Titrations

What animal used most frequently for polyclonal antibodies?

rabbit

What makes polyclonal antibodies poly?

numerous clones of antibodies are produced

Monoclonal antibodies are produced from..

an individual clone of plasma (B) cells

What is the precursor cell to the plasma cell?

B cells

What is a myeloma cell?

cancerous B cell (malignant or neoplastic) that is capable of continuous growth (proliferation); has lost its ability to produce antibodies

Hybrid cells proliferate into clones called…

hybridomas

What are selected to be cultured to produce large amounts of monoclonal antibodies?

hybridomas

T/F monoclonal antibodies need further purification

false

T/F monoclonal antibodies are highly specific

true

T/F majority of primary antibodies used in IHC are polyclonal

false

T/F polyclonal antibodies have higher sensitivity

true

T/F for monoclonal antibodies, target epitope must be unique to an antigen

true

What is the optimal antibody titer

highest dilution of an antibody that results in maximum specific staining with the least amount of background staining

Short incubation times require an antibody with ___

high affinity and high concentrations

Longer incibuation times allow for a __ dilute antibody titer

more

What is the optimal antibody titer?

the highest dilution (lowest concentration) of an antibody that results in maximum specific staining with the least amount of background staining