[ANAL-LAB-MID-01-PT4] OPTICAL ROTATION - POLARIMETER AND UV-VIS SPECTROPHOTOMETER

THIS FLASH CARDS IS ABOUT ANALYSIS; OPTICAL ROTATION - POLARIMETER AND UV-VIS SPECTROPHOTOMETER.

Optical Rotation

This happens when light passes through an optically active substance and its direction is rotated.

• Dextrorotatory (+)

• Levorotatory (-)

Optical Rotation can occur in two ways:

Dextrorotatory (+)

This optical rotation is when light rotates clockwise (to the right).

Levorotatory (-)

This optical rotation is when light rotates counterclockwise (to the left).

Polarimeter

This is a device used to measure optical rotation.

Polarimeter

This devices helps in determining the identity, purity, and quality of substances.

• Identity

• Purity

• Quality

What three aspects of substances can a polarimeter help determine?

• Sugar Solutions

• Turpentine Oil

• Camphor

What are the three (3) common substances tested using a polarimeter?

Spectrophotometry

This is a technique used to measure how much light a chemical substance absorbs at a specific wavelength.

Spectrophotometry

This method is widely used in pharmaceutical analysis to determine the concentration of drugs and other compounds.

200-380 NM

WAVELENGTH RANGES IN SPECTROPHOTOMETRY:

ULTRAVIOLET (UV)

380-780 NM

WAVELENGTH RANGES IN SPECTROPHOTOMETRY:

VISIBLE (VIS)

780-3,000 NM

WAVELENGTH RANGES IN SPECTROPHOTOMETRY:

NEAR INFRARED (NIR)

3.0-15 MICROMETER

WAVELENGTH RANGES IN SPECTROPHOTOMETRY:

MEDIUM INFRARED

15-300 MICROMETER

WAVELENGTH RANGES IN SPECTROPHOTOMETRY:

FAR INFRARED (FIR)

• Light Force

• Monochromator

• Sample Compartment

• Detector

• Display & Processor

What are the five (5) key parts of UV-VIS SPECTROPHOTOMETER?

• Deuterium Lamp

• Halogen Lamp

What are the two types of light source in UV-VIS SPECTROPHOTOMETER?

Deuterium Lamp

This light source provides UV Light (200-400 NM).

200-400 NM

How many in NM is the Deuterium Lamp?

Halogen Lamp

This light source provides UV Light (400-780 NM).

400-780 NM

How many in NM is the Halogen Lamp?

Monochromator

This key part of UV-VIS Spectrophotometer selects a specific wavelength from the light source and eliminates unwanted radiation.

Sample Compartment

This key part of UV-VIS Spectrophotometer holds the sample solution in a cuvette, which is usually made of quartz or glass.

Cuvette

In sample compartment, it holds the sample solution in a?

Quartz or Glass

The cuvette is usually made of?

Detector

This key part of UV-VIS Spectrophotometer detects the light that passes through the sample and converts it into an electrical signal.

Display and Processor

This key part of UV-VIS Spectrophotometer process the signal and displays results as absorbance or transmittance.

Absorbance or Transmittance

The display and processer process the signal and displays results as?