Lecture 32 The Human Genome Project

What were the goals of the human genome project?

The goals of the human genome project included:

• Identify the 20,000-25,000 genes

• Determine the sequence of 3.5 billion bps

• Store the information gathered in databases

• Improve tools for data analysis

• Transfer related technologies to private sector

• Address the ethical, legal and social issues that may arise from the project

Whose DNA was sequenced? How long did it take? How much did it cost?

James Watson’s DNA was sequenced

TAKING 13 YEARS

COSTING $3 billion

What were the different strategies employed by the International Genome Sequencing Consortium
and Craig Venter sequencing teams?

The IGSC utilized MAP BASED SEQUENCING

Craig Venter’s sequencing teams used WHOLE GENOME SHOTGUN SEQUENCING

How much of the human genome is made up of transposable elements? What percentage is protein coding? How many protein coding genes were identified?

50% of the human genome is TRANSPOSABLE ELEMENTS

5% is PROTEIN CODING

30,000-40,000 protein coding regions identified

What is horizontal gene transfer?

Horizontal gene transfer is genetic sharing across species

What is the purpose of the 1000 genome project

The purpose of the 1000 genome project is to build a global reference of human genetic variation