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Positive control
What type of CONTROL is used?
Nutrient broth
What is the culture medium used in this setup?
Enhancement / enrichment medium
What is the characteristic of this culture medium?
Turbidity
Evidence of bacterial growth in culture medium mentioned above
Escherichia coli
Identify the representative organism in this result:
Autoclave, Hot air
What is the physical agent of sterilization in this set-up?
80°C, boiling
80°C - Turbid
Boiling (100°C) - Turbid
Autoclave (121°C) - Clear
Hot Air (180°C) - Clear
What physical agent of disinfection inhibited the organism in above set up?
Bacillus subtilis
Identify the representative organism in this result:
80°C - Turbid
Boiling (100°C) - Turbid
Autoclave (121°C) - Clear
Hot Air (180°C) - Clear
Endospores / presence of endospores
What is responsible for the characteristic result of Bacillus subtilis
80°C - Turbid
Boiling (100°C) - Turbid
Autoclave (121°C) - Clear
Hot Air (180°C) - Clear
Calcium dipicholinate/dipicolinate
What is the composition of the endospore that caused it to have that result?
Escherichia coli was killed by 80°C, boiling, autoclave, and hot air
Control = turbid
80°C = clear
Boiling = clear
Autoclave = clear
Hot air = clear
Interpret the result
Bacillus subtilis was killed by autoclave, and hot air
Control = turbid
80°C = turbid
Boiling = turbid
Autoclave = clear
Hot air = clear
Interpret the result.
Calcium dipicolinate
What substance is responsible for the characteristic result of the test above? (Bacillus subtilis)
Moist heat, dry heat
Based on the set up, how can the agents of sterilization effectively perform its purpose?
Bacteriostatic
Disinfection
Bactericidal
Sterilization
Autoclave
Moist heat
Hot air
Dry heat
Note 
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