protein assays

Which assay isolates a protein with antibodies and pulls down its binding partners from a complex?

Co-Immunoprecipitation (Co-IP)

Which live-cell assay detects protein proximity based on energy transfer between fluorophores?

FRET (Fluorescence Resonance Energy Transfer)

Which technique reconstitutes a split fluorescent protein when two proteins of interest bind?

BiFC (Bimolecular Fluorescence Complementation)

Which method uses enzyme tagging (e.g., BioID or APEX) to label proteins near a target protein?

Proximity Labeling

Which screening system uses transcriptional activation in yeast to detect protein–protein interaction potential?

Yeast Two-Hybrid

Which assay captures in vivo protein–DNA binding by crosslinking, immunoprecipitating, and using PCR to find target DNA?

ChIP (Chromatin Immunoprecipitation)

Which in vitro method shows direct binding between a protein and a DNA sequence via a gel mobility shift?

EMSA (Electrophoretic Mobility Shift Assay)

Which functional assay shows whether a protein activates or represses gene expression using a glowing reporter?

Luciferase Assay

Which gel-based technique quantifies the relative amount of a protein in a sample using antibodies?

Western Blot (WB)

Which plate-based method uses antibodies to quantify protein concentration with high sensitivity?

ELISA (Enzyme-Linked Immunosorbent Assay)

Which antibody-based technique shows where a protein is located in tissue or cells but not its amount?

Immunohistochemistry / Immunofluorescence (IHC/IF)

Which highly sensitive technique quantifies protein concentrations using radioactive labels?

RIA (Radioimmunoassay)

Which assay uses radioactive ATP to determine if a protein phosphorylates a substrate?

Kinase Assay

Which assay detects chemical modifications like phosphorylation, methylation, or acetylation on proteins?

PTM Assay (Post-Translational Modification Assay)

Which technique uses centrifugation to separate cellular components like nuclei or mitochondria?

Cell Fractionation

Which method uses columns to separate proteins based on size or charge for purification?

Chromatography

Which technique uses antibodies bound to beads to isolate a specific protein from a complex sample?

Immunoprecipitation (IP)