Briefly describe the important experimental steps in DNA fingerprinting. Following a major disaster, the bones of four adults and 6 children are discovered in a mass grave. Describe how you could use DNA fingerprinting to determine if any of the individuals belong to a family group, which are males, and which of the adults are the parents. You also have biological samples from a possible maternal grandmother and paternal grandfather. What tests could prove these relationships

Briefly describe the important experimental steps in DNA fingerprinting. Following a major disaster, the bones of four adults and 6 children are discovered in a mass grave. Describe how you could use DNA fingerprinting to determine if any of the individuals belong to a family group, which are males, and which of the adults are the parents. You also have biological samples from a possible maternal grandmother and paternal grandfather. What tests could prove these relationships

what question is this

DNA Fingerprinting and Mass Grave Identification

layout

step/component, description

steps/components

Key Experimental Steps
Family Group Identification
Parent Identification
Sex Determination
Use of Grandparents' DNA
Outcome

Key Experimental Steps

DNA extraction → Restriction enzyme digestion → Gel electrophoresis → Southern blotting → Probe hybridization → Band visualization

experimental step 1

dna extraction

experimental step 2

Restriction enzyme digestion

experimental step 3

Gel electrophoresis

experimental step 4

Southern blotting

experimental step 5

Probe hybridization

experimental step 6

Band visualization

components

Key Experimental Steps
Family Group Identification
Parent Identification
Sex Determination
Use of Grandparents' DNA

Family Group Identification

Compare STR (Short Tandem Repeat) profiles between individuals

Parent Identification

Children should share one allele per locus with each parent

Sex Determination

Amelogenin gene test: X and Y bands for males; single X band for females

Use of Grandparents' DNA

Confirm parent-child links by checking for shared alleles across generations

Outcome

Assign family groups, identify parents, determine sex of individua