LACTATE DEHYDROGENASE (LD) LEC

What is the EC of Lactate Dehydrogenase?

E.C. 1.1.1.27

Other name of LD?

L-Lactate: NAD+ Oxidoreductase

Catalyzes the interconversion of lactate to pyruvate

LACTATE DEHYDROGENASE (LD)

Pyruvate to Lactate conversion is used in what specific cell process?

anaerobic glycolysis

In anaerobic glycolysis how much ATP is produced?

2 ATP per glucose molecule

Coenzyme of LACTATE DEHYDROGENASE (LD)

NAD+ (Oxidized NAD)

Enzymatic Reaction of LD

LACTATE DEHYDROGENASE (LD) is widely distributed in the body. (Y/N)

Yes.

LACTATE DEHYDROGENASE (LD) Tissue souces are in:

– Heart

– Liver

– Skeletal muscle

– Kidney

– Erythrocytes

– Lung

– Smooth muscle

– Brain

Highest levels of LDH are observed in?

Hemolytic Conditions (50X ULN)

What are the 5 isoenzymes of LDH?

LDH 1

LDH 2

LDH 3

LDH 4

LDH 5

Where are each LDH Isoenzyme located?

What is the Major Isoenzyme in our circulation?

LDH 2

Enumerate the LDH Isoenzyme from most numerous to least numerous.

LDH 2

LDH 1

LDH 3

LDH 4

LDH 5

IF LD 1 > LD 2, it suggests?

LD flipped pattern

Suggestive of AMI (Myocardial Infarction)

In Myocardial Infarction, LACTATE DEHYDROGENASE:

• Rise:

• Peak: 4

• Remain elevated:

• Rise: within 12-24 hours after the onset

• Peak: 48-72 hours

• Remain elevated: 10 days