Plasmid Purification and Bacterial Transformation

Flashcards on Plasmid Purification and Bacterial Transformation.

__ are small, circular DNA molecules that exist and replicate separately from the bacteria’s chromosomal DNA.

Plasmids

The pGLO plasmid DNA is used to transform ampicillin- E. coli into ampicillin- E. coli.

sensitive, resistant

Plasmid pGLO carries the gene AmpR, which expresses the enzyme __.

β- lactamase

Plasmid pGLO also carries the GFP gene which expresses the __.

green fluorescent protein

In the experiment, transformed bacteria are resistant to and will glow under light due to GFP.

ampicillin, UV

The basic steps of bacterial transformation includes CaCl₂ treatment to cell walls and selection on bacterial growth medium containing appropriate .

permeabilize, antibiotic

In the transformation procedure, Tube A contains competent E. coli + __ plasmid DNA.

pGLO

In the transformation procedure, Tube B contains competent E. coli + __.

water

In the transformation procedure, Tube C contains competent E. coli + __.

water

Plate A contains LB agar-__-arabinose + competent E. coli + pGLO plasmid DNA.

Ampicillin

In the results, Plate B (LB agar-Ampicillin) shows __ bacterial growth because competent E. coli + water was added.

no

In the results, Plate C (LB agar alone) shows __ non-glowing bacteria + competent E. coli + water.

lawn