Antibody Titration

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6 Terms

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Antibody Titration Principle

measures antibody conc. in serum or compare antigen strength on different red cells samples

  1. Estimating antibody activity in allo-immunized pregnant women

  2. Elucidating autoantibody specificity

  3. Characterizing antibodies with high titer and low avidity (e.g. Knops and Chido/Rodgers systems)

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Antibody titration

  • Determines alloantibody concentration

  • Serum serially diluted, tested against RBCs

    • Includes indirect antiglobulin phase (anti-IgG)

  • Result: reciprocal of endpoint or titer score

  • Critical titer levels set by each lab

  • Titer of 16-32 generally significant

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What specimen is used for Antibody Titration?

Serum or plasma antibody

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What reagents are used for Antibody Titration?

  • Red cells expressing corresponding antibody specificity(ies) in 2-5% saline suspension (uniformity crucial)

  • Saline

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Procedure: Dilution Technique

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Antibody Titer Grading Interpretation:

Observe the highest dilution with 1+ agglutination. Titer is the reciprocal of that dilution (e.g., 32). If agglutination occurs in the most dilute tube, continue dilutions.