Identifying Promoter Elements
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6 Terms
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How were TATA boxes originally identified
By sequence comparison
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What does sequence comparison involve
Identifying common sequences among promoters to generate a consensus sequence
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What is a limitation of sequence comparison
It shows presence of a sequence but not its function
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What are reporter genes
Genes encoding proteins whose expression levels are easy to measure
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Why are enzymes often used as reporter genes
They allow simple enzymatic assays
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How can reporter analysis identify functional promoter elements
By mutating sequences and altering cellular conditions to assess expression changes
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