HISTOPATHOLOGY: CLEARING
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FlashcardsTranslucent (increases refractive index)
Clearing agents makes the tissues __
Glycerin, gum syrup
Clearing agents used in frozen sections
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29 Terms
Translucent (increases refractive index)
Clearing agents makes the tissues __
Glycerin, gum syrup
Clearing agents used in frozen sections
Increase refractive index
Purpose of a clearing agent in frozen sections
Low
Boiling point of clearing agents
Brittleness
Result of overclearing of tissues
Xylene (xylol)
Colorless
Most commonly used in routine procedures
15-30 mins
Clearing time of Xylene (xylol)
Xylene (xylol)
Can be used for celloidin sections
For tissues <5mm thick
Milky
Appearance of xylene when tissues are not completeley dehydrated
Toluene
Substitute for xylene or benzene
Toluene
Tissues do not become excessively hard and brittle even if left 1 day
Not carcinogenic
Benzene
Recommended for urgent biopsies and routine purposes
Milky
Appearance of xylene when tissues are not completeley dehydrated
Toluene
Substitute for xylene or benzene
Toluene
Tissues do not become excessively hard and brittle even if left 1 day
Not carcinogenic
Benzene
Recommended for urgent biopsies and routine purposes
Benzene
Highly toxic
Can cause aplastic anemia
Chloroform
Slower acting than xylene
Used for tissue blocks >1 cm thickness
Chloroform
For hard and tough tissues
Chloroform
Hepatotoxic
Tissues float and do not become translucent
Cedarwood oil
Recommended for CNS tissues and cytology (smooth muscles, skin)
Cedarwood oil
No tissue distortion even when left indefinitely
Milky
Appearance of Cedarwood oil upon prolonged storage
Aniline oil
Not for routine
For embryos, insects, and very delicate tissues
Clove oil
Becomes adulterated
Recommended for skin and smooth muscle
CC4 (Carbon tetrachloride)
Similar but cheaper than chloroform
Methyl salicylate, methyl benzoate
Used for double embedding technique
Carbon disulfide
For smooth muscle
Has a foul odor (rotten eggs)
Terpineol
For delicate materials (eyes)