Lecture on Tissue Preparation and Epithelial Tissue

These flashcards cover key concepts related to tissue preparation and the types of epithelial tissues as discussed in the lecture.

Dehydration is the first step which involves immersing the specimen in ascending concentrations of __.

alcohol (70%-80%-90%-100% ethanol)

The second step in tissue processing is __, where an organic solvent is used to remove alcohol.

clearing

In sectioning, tissue specimens are cut using a __ which slices extremely thin sections.

microtome

Most tissues are cut at around __ thick.

5 μm

To stain lipid-rich structures, one must avoid steps that __ lipids, and use lipid-soluble dyes.

remove

Sudan black is a dye used to reveal lipid-rich structures and can be useful in diagnosing __ diseases.

metabolic

There are __ types of epithelial tissues mentioned in the lecture.

8

Simple columnar epithelial tissue is found in the __.

stomach

Transitional epithelial tissue is found lining the __.

bladder

Pseudostratified columnar epithelial cells line the __.

trachea

Loose connective tissue, also known as __ connective tissue, has a semi-fluid ground substance.

areolar

Fibroblasts, mast cells, and numerous macrophages are types of cells found in __ connective tissue.

areolar