Lab Exam 2 - Mol Cell

cell culture

isolating cells from animal or plant and growing them in a favorable artificial environment

primary culture

stage of the culture after the cells are isolated from the tissue and proliferated under conditions

finite cells

normal cells that divide only a limited number of times before losing their ability to proliferate

continuous cell line

transformed cells that acquire the ability to divide indefinitely

what is an example of normal cells being transformed into immortal cells

carcinogenesis

cell morphology

fibroblast-like, epithelial-like, lymphoblast-like

fibroblast-like cells

are bipolar or multipolar, have elongated shape, and grow attached to a substrate

epithelial-like cells

are polygonal in shape with more regular dimensions and grow attached to a substrate in discrete patches

lymphoblast-like cells

are spherical in shape and usually grown in suspension without attaching to a surface

two systems for growing cells in culture

adherent, suspension

adherent cells

monolayers on an artificial surface

suspension cells

free floating in culture medium

what are hematopoietic cells

blood

what are flask culture vessels

used to grow large batches, contain filtered caps to allow CO2 exchange

what are well plates

used for smaller batches, individual control over the wells

what are petri dishes

used with bacterial culturing, often solid medium used

medium

designed to support the grown of a population cell (amino acids, inorganic salts, vitamins, antibiotics)

laminar flow hoods

provide an aseptic work area while allowing the containment of infectious splashes or aerosols generated by many microbiological procedures

what do laminar flow hoods protect?

clean benches only protect the cultures and the worker and are used if the cultures are harmless, biological safety cabinets are used to protect the cultures from the worker and the worker from the cultures

class I

protects the worker and the environment, but not the cultures

class II

protects the worker, the environment, and the cultures (common)

class III

provides the highest level of protection to worker, environment, and cultures through gas tight design

aseptic technique

• always wear gloves when working inside the hood, gloves are sterilized with 70% ethanol, keep hands inside of the hood

• change pipette tip with each new material and avoid pouring media and reagents directly from bottles or flasks

• never uncover the sterile vessel until you are ready to use it and cover immediately when done

glioblastoma cells

most common type of brain tumor, poor prognosis and high mortality rate, surgery is the major therapeutic method

what body part does glioblastoma effect

the brain

what cell type is polygonal with regular dimensions and grows attached to a substrate in discrete patches

epithelial-like cells

well plates are used to grow small batches of cells and provide individual control over each well

true

what type of sterile work environment protects the worker and the environment, but not the cultures

BSC Class I

good aseptic technique does not require individuals to spray their gloved hands with 70% ethanol prior to entering the hood

false

subculturing

is the transferring of cells from culture to a new vessel with continued growth

confluence

is the percentage of a culture dish that is covered by adhered cells

what is contact initiation

cells stop multiplying (80-90% confluency)