Bovine Pregnancy Detection Methods

What are the two types of preg. detection methods?

Direct (felt or seen) and Indirect (preg. indicating markers)

What are the three things that a detection method should have?

-High sensitivity

-High specificity
-Be simple and inexpensive

What are the 4 methods?

1. Non-return rate

2. Rectal palpation

3. Ultrasonography

4. Blood tests

What does return to estrus equal

= not pregnant

What does non-return on estrus equal

=could be pregnant

Name 5 things that you feel for during a rectal palpation.

1. corpus luteum

2. membrane slip

3. fetus

4. placenta

5. uterine artery palpationovaries

Day of Gestation vs. Crown Rump Length

45

60

90

120

1

2

6

12

What 4 things does a ultrasound show?

1. Pregnancy status as early as day 25

2. fetal sex

3. ovarian function

4. uterine morphology

In what days of gestation are PAGs detected in the maternal blood?

days 22-24 of gestationand can continue to be detected throughout pregnancy.

Who developed RIA in the year of 1986

Sasser(detection in 24 days)

Who developed ELISA in 2005?

Green

What are the commercial preg. tests?

IDEXX- bovine preg. test

IDEXX- rapid visual testand BioTracking - Bovine Pregnancy Test.

What are the 5 pieces of equipment needed for tail vein blood sampling?

1. gloves

2. vacutainer holder

3. vacutainer needle

4. vacutainer blood collection tube

5. bovine tail modeland syringe

What is the red top tube used for?

Plain(clotted)

serum

What are the uses for the red top tube?

-serology

-biochemistry

-down cow

What is the green top tube used for?

-lithium heparin

-plasma 

What are the uses of the green top tube?

-biochemistry

-trace elements

what does the purple top tube do?

EDTA

-CBC