Lab safety

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20 Terms

1
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What should you do if you spill a chemical in the lab?
Report it immediately to your lab instructor or UGTA for proper cleanup instructions.
2
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Are food and drinks allowed in the laboratory?
No, food and drinks are strictly prohibited in the lab.
3
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When should you wash your hands in the lab?
Wash your hands with soap and water before AND after laboratory work.
4
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What is the default PPE you should wear in the lab?
Lab coat, gloves, eye protection, and masks (unless instructed otherwise).
5
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Should you touch specimens or solutions with your fingers, even if gloved?
No, never touch specimens, pipette tips, or solutions with your fingers unless specifically directed.
6
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How should you mix chemicals in a test tube?
Cover the mouth of the tube with parafilm, then gently invert while holding your gloved finger against the parafilm.
7
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What is the correct way to dilute acids?
Always drip acids slowly into water or an aqueous solution—never pour water into concentrated acids.
8
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Can you return unused chemicals to stock bottles?
No, discard unused chemicals into the appropriate chemical waste container.
9
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What should you do to avoid cross-contamination of solutions?
Use a fresh pipette, cylinder, weigh boat, etc., for each reagent when in doubt.
10
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How should you dispose of broken glass?
Place it only in the designated 'broken glass' container.
11
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Where should sharp objects like razors or syringes be disposed of?
In the red 'sharps' container—not in regular garbage or biohazard bins.
12
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How should you clean glassware after use?
Wash with soapy water (Alconox detergent), rinse thoroughly with clean water, and air dry or hand dry.
13
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What should you do with open flames when not in use?
Turn off burners or other flame/heat sources when not in use.
14
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How should you handle hot water baths?
Use tongs or provided materials to avoid direct contact with hot metals or glass.
15
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What should you do with long hair in the lab?
Always tie it back to prevent it from falling into flames, chemicals, or specimens.
16
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Can you take stock solutions to your lab bench?
No, measure the quantity at the stock station and bring only what you need to your bench.
17
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What type of water should you use if the procedure specifies deionized or distilled water?
Use only deionized or distilled water—never assume tap water is acceptable.
18
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What should you do if you’re unsure how to use equipment or reagents?
Ask your lab instructor or UGTA for guidance.
19
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What should you do if you need to leave the lab during a protocol?
Inform your instructor or UGTA, remove PPE, dispose of gloves properly, and put PPE back on when you return.
20
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Is this list exhaustive for lab safety precautions?
No, always check the lab manual and bench for additional precautions or procedures.