research paper #2

what is hMEC/D3?

human blood brain microvascular endothelial cells

what is MDCKII-BCRP?

canine renal cells that overexpress human breast cancer resistance proteins

what is MDCKII-MRP2?

canine epithelial cells that overexpress human multidrug resistance-associated protein 2 (ABCC2)

what is the tubule system?

killifish isolated kidney proximal tubules

what is the substrate and inhibitor for Bcrp?

sub: BodIPYFL

inhib: Ko143

what is the substrate and inhibitor for P-gp?

sub: Rh123

Inhib: psc833

what is the substrate and inhibitor for Mrp2?

sub: Texas Red

inhib: MK-571

what does figure 2 show?

PSC833 (p-GP inhibitor) significantly increased intracellular Rh123 flourecense demonstrating expected inhibitory effect

purpose of this study?

to find out if toxicity of Al had an effect on efflux activity of ABC transporters: BCRP, P-gp, Mrp2 in MDCKII cells and isolated kidney tubules.