Competency Review Advanced Micro 3

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1
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Colony and microscopic morphology of: Penicillium
Colony and microscopic morphology of: Penicillium
Microscopic: Septate hyphae, branched conidiophores with 2 branches, flask shaped phialides with chains of round conidia - brush like

Colony: flat, powdery, blue green with white edge
Microscopic: Septate hyphae, branched conidiophores with 2 branches, flask shaped phialides with chains of round conidia - brush like 

Colony: flat, powdery, blue green with white edge
2
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Colony and microscopic morphology of: Fusarium,
Colony and microscopic morphology of: Fusarium,
Microscopic: septet hyphae, large multi-celled sickle shaped macroconidia on simple conidiophores. small 1-2 celled tear-drop shaped microconidia

Colony: flat, pink to violet with light edge
Microscopic: septet hyphae, large multi-celled sickle shaped macroconidia on simple conidiophores. small 1-2 celled tear-drop shaped microconidia

Colony: flat, pink to violet with light edge
3
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Colony and microscopic morphology of: Gliocladium
Colony and microscopic morphology of: Gliocladium
Microscopic: Branching conidiophores with flask-shaped phialides like penicillium, microconidia cluster in ball like a golf ball

Colony: White at first, center becomes dark green, reverse: white
Microscopic: Branching conidiophores with flask-shaped phialides like penicillium, microconidia cluster in ball like a golf ball

Colony: White at first, center becomes dark green, reverse: white
4
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Colony and microscopic morphology of: Paecilomyces
Colony and microscopic morphology of: Paecilomyces
Microscopic: branching conidiophores with flask-like phialides in pairs or brush-like groups, long chains of oval microconidia, resembling penicillium

Colony: yellowish brown, pink, white with reverse off-white, light yellow, pale brown
Microscopic: branching conidiophores with flask-like phialides in pairs or brush-like groups, long chains of oval microconidia, resembling penicillium 

Colony: yellowish brown, pink, white with reverse off-white, light yellow, pale brown
5
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Colony and microscopic morphology of: Bipolaris
Colony and microscopic morphology of: Bipolaris
Microscopic: elongated conidiophores, oblong conidia with 3 to 5 separation

Colony: Gray, brown, or black with brown. Reverse black.
Microscopic: elongated conidiophores, oblong conidia with 3 to 5 separation 

Colony: Gray, brown, or black with brown. Reverse black.
6
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Know the names of the structures found in Penicillium
Know the names of the structures found in Penicillium
Septate hyphae, branched conidiophores w/ secondary branches, flask shaped phialides w/ chains of round conidia
Septate hyphae, branched conidiophores w/ secondary branches, flask shaped phialides w/ chains of round conidia
7
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Know the names of the structures found in Fusarium
Septate hyphae, large multi-celled sickle-shaped macroconidia on simple conidiophores, small 1-2 celled tear-drop-shaped microconidia
Septate hyphae, large multi-celled sickle-shaped macroconidia on simple conidiophores, small 1-2 celled tear-drop-shaped microconidia
8
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Microscopic morphology of: Alternaria
Microscopic morphology of: Alternaria
septate hyphae and dark
large conidia, club shaped
septate hyphae and dark
large conidia, club shaped
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Microscopic morphology of: Curvularia
Microscopic morphology of: Curvularia
Septate hyphae
Large conidia with up to 4 cells
central cell swollen - curved
Septate hyphae 
Large conidia with up to 4 cells 
central cell swollen - curved
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Microscopic morphology of: Scopulariopsis
Microscopic morphology of: Scopulariopsis
short conidiophores with round, rough chains of conidia
short conidiophores with round, rough chains of conidia
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Gram stain results for: Bacillus anthracis
Gram Positive Rod
Gram Positive Rod
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Gram stain results for: Francisella tularensis
Tiny Gram Negative Coccobacillus
Tiny Gram Negative Coccobacillus
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Gram stain results for: Yersinia pestis
Gram Negative Rods - may be bipolar stained
Gram Negative Rods - may be bipolar stained
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The colony morphology of Bacillus anthracis
The colony morphology of Bacillus anthracis
Aerobic, 4-8hr growth
No growth on MacConkey (GPR)
Flat colonies with irregular edges
Non-hemolytic on BAP
Sticky colonies
Aerobic, 4-8hr growth
No growth on MacConkey (GPR)
Flat colonies with irregular edges
Non-hemolytic on BAP
Sticky colonies
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Which of these are you most likely to come across in the southwest?
Yersinia pestis (plague)
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Know the diseases caused by each organism: Bacillus Anthracis
Anthrax
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Know the diseases caused by each organism: Francisella Tularensis
Tularemia
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Know the diseases caused by each organism: Yersinia pestis
Plague
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Things to consider determining if you have a bioterrorism organism
Patient history/travel history

Symptoms

Gram stain results
- GP, small GNC, faintly staining GNR

Growth and/or no growth on routine culture plates
- Slow growth on blood agar plate
- Slow or no growth on MacConkey plate