lec 9 - Genome Integrity maintenance and cancer development

what are the 4 DNA repair mechanisms?

what are the 2 types of TSGs involved in genome integrity?

how do elevated mutation rates affect cancer?

what are the two underlying mechanisms leading to genetic instability?

describe the 2 ways DNA damage can occur

how does DNA pol proofread?

how does DNA break in replication

how does deamination from C to U cause DNA damage?

how does deamination from C to T cause DNA damage?

how does oxidation lead to DNA damage?

how does inflammation cause DNA damage?

compare transversions and transitions

how are pyrimidine dimers formed? how does this effect the DNA

what are the consequences of pyrimidine dimer formation during replication?

how does alkylation cause DNA damage?

describe how DNA adducts form

how do DNA adducts cause DNA damage?

how does Aflatoxin become carcinogenic in the liver?

what are the 2 DNA repair mechanisms?

describe base excision repair (BER)

describe nucleotide excision repair (NER)

describe Xeroderma pigmetosum and how inherited defects in NER cause cancer

what are the 2 types of mismatches?

what are the 2 types of mismatch repair proteins? and how do they work?

how do defects in mismatch repair affect microsatellites?

describe how inherited defects in MMR cause Hereditary non-polyposis

what can cause dsDNA breaks? what can these lead to?

what are BRCA1 and BRCA2’s role in DNA damage repair?

describe homology directed repair

how are BRCA1 and BRCA2 involved in homology directed repair

describe non-homologous end joining (NHEJ)