fluorescent stains
absorbs light to re-emit at longer wavelengths.
makes images brighter.
immunoflurescent
fluorescent attached to specific chemicals.
tracks the movement of chemicals in a cell.
freeze fracture
cells are frozen in a very cold propane, then split apart and prepared.
allows cell structures to become visible.
cryogenic electron microscope
used for protein structure research.
magnification (def.)
how much bigger the image appears than the actual size.
field of view (fov)
visible circle seen through the ocular.
resolution
ability to distinguish 2 points as seperate or distinct.
magnification (measure)
image size / actual size
image size
magnification x actual
actual size
image / magnification
unicellular
all metabolic functions in one cell.
multicellular
cells are specialized, organized and dependent.
organized into compartments.
prokaryotes
have no membrane-enclosed compartments.
eukaryotes
have membrane-enclosed compartments called organelles, such as the nucleus.
advantages in eukaryotic cells being compartmentalized
Efficiency of metabolism
Localized conditions
Toxic/damaging substances can be isolated
The numbers & locations of organelles can be changed
all living things can do…
Metabolism
Response
Homeostasis
Growth
Excretion
Rreproduce
Nutrition
plasmids
dna molecule that is separate from, & can replicate independently of, the chromosomal DNA.
prokaryotes reproduce…
asexually. they clone themselves: copy dna, then split.
name the different prokaryote(s) and eukaryote(s)…
prokaryote: bacteria
eukaroyote: animal, plant, fungi, protist
cell theory
all living things are made of cells, “basic life units.” new cells arise from pre-existing cells.