2 - Expt. 2 Cyanide Detection

seeds

all cassava tissues contain cyanogenic glycoside except ____

leaves and root

The highest cyanide levels are found in the ____ and ____

hydrogen cyanide, cyanogen chloride
2,500-5,000 and 11,000

Airborne release of cyanide gas, in the form of ____ or ____, would be expected to be lethal at levels of _____ mg•min/m3 and ____ mg•min/m3

100-200

When ingested as sodium or potassium cyanide, the lethal dose is ___ mg.

1. cyanide antidote kit

2. hydroxocobalamin

2 options for cyanide poisoning

• amyl nitrite

• sodium nitrate

• sodium thiosulfate

3 components of cyanide antidote kit

amyl nitrite

comes in pearls that must be broken down and inhaled to initiate production of methemoglobin that attracts the cyanide ions

intravenous sodium nitrate

further increases methemoglobin production and liberates it from binding to the cytochrome oxidase

intravenous sodium thiosulfate

converts cyanmethemoglobin to excretable thiocyanate

cyanocobalamin (vitamin B12)

The cobalt-containing substance hydroxocobalamin becomes _____ after binding to cyanide which is then excreted in the urine.

aerobic cell metabolism

The toxicity of cyanide is linked mainly to the cessation of ______

ferric ions cytochrome oxidase three (III)

Cyanide reversibly binds to the _____ within the mitochondria.