LACTATE DEHYDROGENASE (LDH) DETERMINATION

What is the method?

Kinetic method by Buhl and Jackson, Modified by Wacker

The enzyme lactate dehydrogenase is distributed in tissues particularly the _________, __________, ___________, _____________.

heart, liver, muscle and kidney

The enzyme found in circulation is a mixture of five isoenzymes based on their mobility. Elevated serum levels of LDH are observed in _________________, _____________, ________________, ______________, ________________, _______________.

myocardial infarction, liver disease, renal disease, certain forms of anemia, malignant diseases and progressive muscle dystrophy

Lactate to pyruvate method offers a number of advantages such as ________________, _________________, ________________.

better reagent stability, elimination of pre-incubation in the procedure, rate of reaction being linear over a wide range and less contamination of NAD with inhibiting products

The rate of NADH formation measured at _____________ is directly proportional to the activity of serum LDH.

340 nm

Principle of Reaction

Lactate dehydrogenase does not act on __________ and only __________ acts as the coenzyme.

D-lactate and only NAD

The pyruvate formed will proceed to the __________________ for generation of energy

Embden-Meyerhoff pathway