Molecular Biology 1 Exam 2 - Ch. 4-6 (Robert Borgon, UCF) Proteins

Over 300 diseases are related to what mistake?

Protein Misfolding

WD40 domain

protein-protein interaction with stable propeller-like platform

EF-hand domain

Calcium Binding

i.e. Calmodulin

What is the only domain that is not involved in a protein-protein interaction?

EF-hand domain

SNARE domain

protein-protein interaction in intracellular fusion

Bromo domain

protein-protein interaction in chromatin remodeling

i.e. acetylated lysine

Chromo domain

protein-protein interaction in chromatin remodeling

SH2 domain

binds phosphotyrosine

SH3

binds proline rich sequences

Zinc finger proteins

Motor neuron survival, differentiation, and axon growth

A defect in which protein will result in spinal muscular atrophy?

Zinc Finger Protein 1

Advantages of multi-domain proteins

faster folding time

stable

each compartment has a special role

regulation of production

substrate channeling

tyrosine kinase

An enzyme that catalyzes the transfer of phosphate groups from ATP to the amino acid tyrosine on a substrate protein.

c-Abl

__________ is found in p53 and recognizes methylated histones.

Tudor domain

Levels of Glycolysis

Glucose --> Glucose 6-phosphate -->Fructose 6-phosphate --> Fructose 1,6-bisphosphate --> glyceraldehyde 3-phosphate --> 1,3-Bisphosphoglycerate --> 3-phosphoglycerate -->2-phosphoglycerate --> Enolase --> Phosphoenolpyruvate --> Pyruvate

What is located in the Kinase domain from 32 to 250 bp?

Serine 32

What is located in the Phosphatase domain from 250 to 470 bp?

Serine 32-P

fatty acid synthase

major enzyme complex in fatty acid synthesis

found in the cytosol

becomes rapidly induced in the liver following a high meal in carbohydrates - because of the elevated insulin levels

contains

acyl carrier protein (ACP)

pantothenic acid - vitamin B5

NADPH - needed to reduce acetyl groups added to the fatty acid

malonly CoA attaches to the ACP - activating it

carbonyl group goes to a hydroxyl group and CO2 is lost

(NADPH --> NADP+)

dehydration - water is lost

double bond is reduced and fatty acid is formed

(NADPH)

ACP attaches to Coenzyme A by what what biological tether?

Ser-41 (phosphodiester bond)

Why in fatty acid synthase do bacterial cells differ from mammalian cells?

Bacteria - separate proteins

Mammals - single polypeptide has domains due to gene fusions

In yeast, there is (a/an) (increased/decreased) amount of robustness and adaptability in genes with more duplications.

increased

humans have 23 duplications not in other primates

Vision in color relates to what cell types?

photopsins

photopsins

short, medium, long wave sensitivity in cones

Dichromats = ?

Trichromats = ?

new world monkeys

old world monkeys

T/F: Gene duplications result in NEW domains/bundles.

True

How large is the average human protein?

~42 kDA (375 aa)

What is the protein titin involved in?

i.e. Connectin

muscle elasticity, resting tension, spring like

What is the longest "word" in English?

C132983 H211861 N36149 O40883 S693

Takes ~ 3-6 aa/sec in eukaryotes

What does cooperativity or allostery of the protein quaternary structure indicate?

Response Time