Intro Genetic Engineering Flashcards

Flashcards for Genetic Engineering lecture review.

Genetic Engineering

The use of molecular tools to manipulate and alter the genetic material of organisms.

Topics in Genetic Engineering

Basic laboratory techniques, Basic enzymology for in vitro DNA manipulation, Molecular Cloning, Gene Libraries, Expression of cloned genes, Modification of DNA sequences: mutagenesis and gene editing, Synthetic Biology

Restriction Enzymes

Cutting DNA at specific sequences.

DNA Ligases

Joining DNA fragments together.

PCR (Polymerase Chain Reaction)

A method to amplify DNA in vitro.

Molecular Cloning

Using a biological system to produce many identical copies of a DNA sequence.

Gene Library

A collection of DNA fragments that represent the entire genome of an organism.

Transcription

The process of making RNA from a DNA template.

Translation

The process of making protein from an RNA template.

Bioinformatics

The use of computational tools to analyze biological data.

Four steps in DNA cloning

Source of DNA, Joining to vector, Introduction into host cell, Selection of cloned sequence

Cloning strategies

Clone gene, clone bank (library), clone cDNA