Dengue IgG / IgM Test

Dengue Virus is transmitted by Aedes aegypti & Aedes albopictus mosquitoes, are widely distributed throughout the tropical & subtropical areas of the world.

Dengue Virus is transmitted by - mosquitoes, are widely distributed throughout the tropical & subtropical areas of the world.

Dengue is an important arthropod-borne viral disease due to its high morbidity & mortality in humans

Dengue is an important arthropod-borne viral disease due to its high -in humans

• Dengue Virus 1

• Dengue Virus 2

• Dengue Virus 3

• Dengue Virus 4

4 Known Distinct Serotype of Dengue Virus

Dengue in children, infection is often sub-clinical or causes a self- limited febrile disease.

Dengue in children, infection is often - or causes a - disease.

Dengue

If the patient is reinfected with a different serotype, risk of a more severe disease increase, such as dengue hemorrhagic fever or dengue shock syndrome.

Dengue

If the patient is reinfected with a different serotype, risk of a more severe disease increase, such as - or -

NS1 Antigen

- Is highly-conserved glycoprotein that is present at high concentration in the sera of dengue-infected patients, during the early clinical phase of the disease.

1-9 days

NS1 Antigen

- Found in specimen from primary or secondary dengue-infected patients between _ after onset of fever.

NS1 Antigen

- IgM usually become detectable until 5-10 days after the onset of illness in cases of primary dengue infection & until 4 – 5 days after onset of illness in secondary infections.

NS1 Antigen

- IgM usually become detectable until - days after the onset of illness in cases of primary dengue infection & until 4 – 5 days after onset of illness in secondary infections.

NS1 Antigen

- IgM usually become detectable until 5-10 days after the onset of illness in cases of primary dengue infection & until 4 – 5 days after onset of illness in secondary infections.

NS1 Antigen

- IgM usually become detectable until 5-10 days after the onset of illness in cases of primary dengue infection & until - days after onset of illness in secondary infections.

F

- In primary infections, IgG appears by the 14th day & persists for life.

NS1 Antigen (t/f)

- In primary infections, IgG appears by the 16th day & persists for life.

NS1 Antigen

- In secondary infections, IgG levels rise for 1–2 days after the onset of symptoms & an IgM response is induced 20 days after infection.

NS1 Antigen

- In secondary infections, IgG levels rise for - days after the onset of symptoms & an IgM response is induced - days after infection.

Dengue IgG/IgM test

is designed to simultaneously detect & differentiate IgG & IgM antibodies to dengue virus (DEN 1,2, 3 and 4) in human serum, plasma.

recombinant Dengue envelope proteins

Dengue IgG/IgM test also can detect all 4 dengue serotypes by using a mixture of -. Both the mouse monoclonal anti-human IgG & IgG on the membrane & the Dengue virus envelope protein gold colloid will react specifically with IgG & IgM antibody to Dengue virus from human serum or plasma.

Dengue IgG/IgM test also can detect all 4 dengue serotypes by using a mixture of recombinant Dengue envelope proteins. Both the mouse monoclonal anti-human IgG & IgG on the membrane & the Dengue virus envelope protein gold colloid will react specifically with IgG & IgM antibody to Dengue virus from human serum or plasma.

Dengue IgG/IgM test also can detect all 4 dengue serotypes by using a mixture of recombinant Dengue envelope proteins. Both the - & IgG on the membrane & the - will react specifically with IgG & IgM antibody to Dengue virus from human serum or plasma.

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Dengue IgG/IgM test device has 3 pre-coated lines, “G” (dengue IgG Test Line), “M” (dengue IgM test line) & “C” (control Line) on the surface of the device. All 3 lines in result window are not visible BEFORE applying any specimen.

Dengue IgG/IgM test device has 3 pre-coated lines, “G” (dengue IgG Test Line), “M” (dengue IgM test line) & “C” (control Line) on the surface of the device. All 3 lines in result window are visible BEFORE applying any specimen. (t/f)

Control line

is used for procedural control and should always appear if the test procedure is performed properly & the test reagents of control line are working.

F

A purple “G” & “M” lines will be visible in the result window if there are enough IgG &/or IgM antibodies to Dengue virus in the specimen. (t/f)

A purple “G” & “M” lines will not be visible in the result window if there are enough IgG &/or IgM antibodies to Dengue virus in the specimen. (t/f)

F

If IgG &/or IgM antibodies to Dengue virus are NOT PRESENT in the specimen, there is no color appears in “G” &/or “M”.

If IgG &/or IgM antibodies to Dengue virus are NOT PRESENT in the specimen, a color appears in “G” &/or “M”. (t/f)

Dengue IgG/IgM Test

When a specimen is added to the specimen well, Anti-dengue IgGs & IgMs in the specimen will be captured by the relevant anti-human IgG &/or anti-human IgM immobilized in two test lines across the test device. Mouse monoclonal anti-dengue Ab-colloidal gold conjugates will react with dengue virus in virus pad & forms a complex of antibodies-antigen. As this complex migrates along the length of the test device by capillary action, it will react with Anti-Dengue IgGs & IgMs in test lines & generate a colored line

Dengue IgG/IgM Test

When a specimen is added to the specimen well, Anti-dengue IgGs & IgMs in the specimen will be captured by the relevant anti-human IgG &/or anti-human IgM immobilized in two test lines across the test device.- will react with dengue virus in virus pad & forms a complex of antibodies-antigen. As this complex migrates along the length of the test device by capillary action, it will react with Anti-Dengue IgGs & IgMs in test lines & generate a colored line

Dengue IgG/IgM Test

When a specimen is added to the specimen well, Anti-dengue IgGs & IgMs in the specimen will be captured by the relevant anti-human IgG &/or anti-human IgM immobilized in two test lines across the test device. Mouse monoclonal anti-dengue Ab-colloidal gold conjugates will react with dengue virus in virus pad & forms a complex of antibodies-antigen. As this complex migrates along the length of the test device by capillary action, it will react with Anti-Dengue IgGs & IgMs in test lines & generate a colored line

Dengue IgG/IgM Test

When a specimen is added to the specimen well, Anti-dengue IgGs & IgMs in the specimen will be captured by the relevant anti-human IgG &/or anti-human IgM immobilized in two test lines across the test device. Mouse monoclonal anti-dengue Ab-colloidal gold conjugates will react with dengue virus in virus pad & forms a complex of antibodies-antigen. As this complex migrates along the length of the test device by - , it will react with Anti-Dengue IgGs & IgMs in test lines & generate a colored line

Dengue IgG/IgM test

is a solid phase immunochromatographic assay for the rapid, qualitative & differential detection of IgG & IgM antibodies to dengue virus in human serum/plasma.

Dengue IgG/IgM test

This test is intended for professional used to aid in the presumptive diagnosis between primary & secondary dengue infection.

Dengue IgG/IgM test

This test provides ONLY A PRELIMINARY TEST RESULT. Therefore, isolation of virus, antigen detection in fixed tissues, RT-PCR & serologic test like haemagglutination-inhibition test, more specific alternative diagnosis method must be used in order to obtain a confirmation of dengue virus infection.

• Micropipettes

• Protective gloves

• Timer

• Phlebotomy kit

• Salmonella typhi IgG/IgM Fast test kit

• Biohazard container

• Specimen

• Serum

• Plasma

Dengue IgG/IgM test Materials

Test Procedure of Dengue IgG/IgM test

1. Bring all kit components & specimen to reach room temperature (between 15C & 30°C) prior to opening & testing.

Test Procedure of Dengue IgG/IgM test

1. Bring all kit components & specimen to reach room temperature (between - °C) prior to opening & testing.

Test Procedure of Dengue IgG/IgM test

2. Remove the test device from foil pouch, & place it on a flat, dry surface. Label the test device with a patient identifier.

Test Procedure of Dengue IgG/IgM test (t/f)

2. Remove the test device from foil pouch, & place it on a flat, dry surface. Label the test device with a -.

Test Procedure of Dengue IgG/IgM test

3. Using a micropipette: Dispense 5uL of serum/plasma specimen into the square specimen well.

Using a capillary pipette: Draw serum/plasma to black line (5uL) then dispense into the square specimen well.

Test Procedure of Dengue IgG/IgM test

3. Using a micropipette: Dispense -uL of serum/plasma specimen into the square specimen well.

Using a capillary pipette: Draw serum/plasma to black line (-uL) then dispense into the square specimen well.

4. Dispense 4 drops (about 90–120uL) of assay diluent into the round assay diluent well.

Test Procedure of Dengue IgG/IgM test

4. Dispense - drops (about - uL) of assay diluent into the round assay diluent well.

T

5. Interpret test results in 15–20 minutes.

Caution: Do not read test results after 20 minutes, reading after 20 minutes can yield false results.

Test Procedure of Dengue IgG/IgM test (t/f)

5. Interpret test results in 15–20 minutes.

Caution: Do not read test results after 20 minutes, reading after 20 minutes can yield false results.

F

5. Interpret test results in 15–20 minutes.

Caution: Do not read test results after 20 minutes, reading after 20 minutes can yield false results.

Test Procedure of Dengue IgG/IgM test

5. Interpret test results in 15–20 minutes.

Caution: Do not read test results after 20 minutes, reading after 20 minutes can yield positive results. (t/f)

+

Test Interpretation of Dengue IgG/IgM test

The presence of any test line, no matter how faint, the result is considered .

IgG +

Test Interpretation of Dengue IgG/IgM test

The presence of two line (“C” & “G”) in the result window, regardless of which line appears first, indicates that the specimen is - antibodies to dengue virus.

IgG +

Test Interpretation of Dengue IgG/IgM test

This suggests secondary or past dengue infection.

F

Test is positive even if “G” line is faint.

Test Interpretation of Dengue IgG/IgM test (t/f)

Test is negative even if “G” line is faint.

IgM +

Test Interpretation of Dengue IgG/IgM test

The presence of 2 colored line (“C” and “M”) in the result window, regardless of which line appears first, indicates that the specimen is - antibodies to dengue virus.

IgM +

Test Interpretation of Dengue IgG/IgM test

This suggests primary dengue infection.

IgM +

Test Interpretation of Dengue IgG/IgM test

Test is - even if “M” line is faint.

IgG & IgM +

Test Interpretation of Dengue IgG/IgM test

The presence of 3 colored line (“C”, “M” & “G”) in the result window, regardless of which line appears first, indicates that the specimen is - for both - antibodies to dengue virus.

IgG & IgM +

Test Interpretation of Dengue IgG/IgM test

This suggests a late primary & early secondary or past dengue infection.

IgG & IgM +

Test Interpretation of Dengue IgG/IgM test

Test is - even if “M” and “G” line are faint.

–

Test Interpretation of Dengue IgG/IgM test

Presence of only Control Line “C” in the result window indicates that IgG & IgM antibodies to dengue are not present in the specimen or are present below the detectable levels.

Ir result is invalid, retest 3–5 days if dengue infection is suspected.

Test Interpretation of Dengue IgG/IgM test

Ir result is –, retest - days if dengue infection is suspected.

Invalid Result

Test Interpretation of Dengue IgG/IgM test

No control line “C” in the result window means the test is -, whether or not the Test Line “M” and/or “G” is present.

Invalid Result

Test Interpretation of Dengue IgG/IgM test

The instructions may not have been followed correctly or the test may have been compromised.

Invalid Result

Test Interpretation of Dengue IgG/IgM test

When an invalid result is obtained, it is recommended that the specimen be retested using a new device.