What macromolecule is ATP?
Nucleotide
What properties of ATP make it suitable for energy transfer?
Soluble in water
Stable at neutral pH
Controlled movement due to inability to diffuse out of a cell
Third phosphate can easily be removed
Hydrolyzing ATP to ADP and phosphate releases only a small amount of energy, just enough for cell to use
What 3 main activities require energy from ATP?
Synthesizing macromolecules
Active transport against the concentration gradient
Movement within the cell and of the cell as a whole
Where can energy come from?
Cell respiration, photosynthesis, chemosynthesis (oxidizing inorganic substances)
Oxidation
The loss of electrons
What happens during respiration?
Carbon compounds are oxidized to create energy, which is used to produce ATP
What main carbon compounds are oxidized in respiration?
Glucose and fatty acids
Gas exchange
Oxygen enters cells through the plasma membrane while carbon dioxide exits cells
Aerobic respiration equation
C6H12O6 +O2 = CO2 + H2O + ATP (catabolic reaction)
Anaerobic respiration in humans equation
C6H12O6 = lactate + ATP
Anaerobic respiration in yeast equation
C6H12O6 = ethanol + CO2 + ATP
Advantage of anaerobic respiration
Produces ATP rapidly over a short period of time
Oxygen debt
The oxygen that it takes to break down lactate buildup after anaerobic respiration has occurred
Reduction
Gain of electrons, happens at the same time as oxidation
NAD
Electron carrier that can easily accept and lose electrons. Links oxidation and reduction in cells during cellular respiration
How are substances oxidized in respiration?
2 hydrogen atoms are removed (one electron and one proton)
NADH
NAD after it has accepted 2 electrons and one of the two protons from oxidized substances
Glycolysis
First stage of cellular respiration, glucose or monosaccharide is the reactant. In the cytoplasm, glucose is converted to pyruvate by a chain of reactions. Produces a small yield of 2 atps per glucose
How is reduced NAD converted to NAD in anaerobic reactions?
Hydrogen is transferred from NADH to pyruvate, converting it into lactic acid (lactic fermentation)
How is anaerobic respiration in yeast different from normal anaerobic respiration?
Reduced NAD is converted to NAD through conversion of pyruvate into ethanol and CO2. (ethanol/alcoholic fermentation)
Link reaction
Connects glycolysis and the krebs cycle; pyruvate is oxidized into CO2 and H2O which yields much more ATP than anaerobic respiration. Initial reaction is conversion of pyruvate to a 2-carbon acetyl group. Takes place in the matrix of mitochondria
3 steps of a link reaction
Decarboxylation: CO2 is removed,3-carbon pyruvate is converted to 2-carbon molecule
Oxidation: 2 electrons removed and accepted by NAD to produce reduced NAD
Binding: acetyl group is bound to coenzyme A, a carrier molecule
Krebs cycle
Acetyl groups are oxidized in a series of reactions that take place in the matrix. Acetyl groups are fed to the cycle when coenzyme A transfers the group to oxaloacetate, creating citrate. The goal of the reaction is to remove two carbons from citrate to produce oxaloacetate again through decarboxylation reactions where CO2s are removed. Released energy is held by the removed electrons, which are carried by NAD or FAD to the electron transport chain.
Electron transport chain
Proteins acting as election carriers receive and transfer the electrons from the Krebs cycle.
How does substrate concentration affect rate of respiration?
As the amount of substrate increases, so does the rate of respiration, until it plateaus at the maximum activity.
How does yeast concentration affect rate of respiration?
As yeast concentration increases, so does the rate of respiration until it plateaus at maximum activity.
How does pH affect rate of respiration?
Starts off low, then gradually increases until pH reaches 4.7, and then decreases as pH gets more basic. Denatures around pH of 6
How does the temperature affect respiration rate?
Graph looks like a craggy mountain; gradually goes up until it reaches 20C then goes up fast until it peaks at around 43C then goes down steeply then more gradually.