NUCLEIC ACIDS & ENZYME

Nucleic acids

Are made up of long lasting chains of nucleotides connected to one another by phosphodiester bonds.

Phosphodiester bonds

Nucleic acids are made up of long lasting chains of nucleotides connected to one another by __

Nitrogenous bases, sugar, phosphate

Upon hydrolysis, nucleotides yield these

DNA, RNA, nucleus

These are the components of the genetic materials __ and __ , and are localized in the __ of the cell

Purine, pyrimidine

These are the two kinds of nitrogenous bases:

Adenine, guanine

These are the two kinds of purine

Cytosine, thymine

These are the two kinds of pyrimidine

Ribose, deoxyribose

The sugar components of nucleic acids are of two kinds, these are pentose sugars

Phosphoric acids

The __ are attached to the 5th carbon of the sugar

Deoxyribonucleic acid

The genetic material for living organisms, most cells contain these in the form of chromosomes located in the nucleus, in this experiment, there will be a purification of this from the living cells of plant tissue such as lentil beans or peas

Papain

The first step in the purification of DNA from plants will be to digest the cell wall with an enzyme called __

Papain

A naturally occuring enzyme used in some meat tenderizers

Sulfuric Acid

H2SO4

Hydrochloric acid

HCl

Sodium Hydroxide

NaOH

Nitric acid

HNO3

Copper Sulfate

CuSO4

Trichloroacetic acid

CCl3COOH

Trichloroacetic acid

TCA

Hydrogen Peroxide

H2O2

Enzyme activity

A measure of the rate at which an enzyme converts substrate to products in a biochemical reaction

Temperature, pH, substrate concentration, enzyme concentration

Four factors that affect enzyme activity