Molecular Biology

**Lysis buffer** and **Proteinase K**

The cell and nucleus are broken open to release the DNA inside using detergents and enzymes

**Precipitation**

Separation of DNA from its cellular debris using **isopropanol or ethanol.**

**Purification**

DNA separated from aqueous phase can be rinsed with **alcohol or TE buffer**

**Tris**

•buffer to maintain pH of the solution

**SDS (Sodium dodecyl sulfate)**

•to disrupt cell membranes

**EDTA (Ethylenediaminetetraacetic Acid)**

•to inhibit enzymes such as DNase I

**Proteinase K**

•An enzyme used to remove proteins

chaotropic salts

DNA binds to silica in the presence of

extract once with phenol

•The standard way to remove proteins from nucleic acids solutions is to ____, once with a 1:1 mixture of phenol and chloroform, and once with chloroform.  This procedure takes advantage of the fact that deproteinization is more efficient when two different organic solvents are used instead of one.