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Firmicutes
Gram-positive bacteria (Staphlococcus, Bacillus)
Proteobacteria
Gram-negative bacteria (pseudomonas, E coli)
Terpenoids
hydrocarbon chains in archaea (from isoprene)
Ether links in archaea
hydrocarbons to glycerol
Hopanoids
reinforce cell membrane
Vancomycin
antibiotic; prevents cross-bridging by binding to terminal alanine dipeptide
Beta-lactamase
penicillin resistance; cleaves penicillin
Lipid A
in LPS; short chain fatty acid linked to glucosamine dimer
Core polysaccharide
in LPS; 5 sugars
O polysaccharide
in LPS; linked to core polysaccharide
Z ring
formed by FtsZ for cell septation
Replisome
DNA polymerase complexes; make DNA at each fork
How many DNA polymerases per replisome?
2 (leading + lagging)
End of replication
triggers Z-ring formation by FtsZ
Cocci arrangement
determined by septation orientation
Metachromatic granule
storage; inorganic phosphate (ADP → ATP)
Sulfur granules
oxidize H2S → S0 (insoluble granules)
Lipid inclusion
storage; PHB (polyhydroxybutyrate)
Fastidious bacteria
need defined (synthetic, minimal) media
Log phase
most active/growth; cells enlarge
Late log phase
growth slows down
Death phase
exponential decline
Dormancy/persistence
growth arrested cells remain viable and provide energy
When are fed-batch cultures fed?
mid-log phase
Quorum sensing
cell communication triggering exopolysaccharide formation; cell density dependent and nutrient driven
Endospore forming bacteria:
Bacillus and Clostridium
Viroid effect
alters gene expression in plants
Viral shunt
viral infection turns hosts into organic/inorganic molecules
Virus that require RDRP
Group 3, 4, 5 (ds RNA, + ssRNA, - ssRNA)
Virus that use host RNA polymerase
Group 1, 2, and 6 (ds DNA, + ssDNA, retrovirus)
Lambda phage
temperate phages with lysogenic cycle
Prophage
temperate phage integrates into cell chromosome as a prophage
Virulent phage
only carry out lytic cycle (T4)
Genetic resistance
altered receptor proteins
Restriction endonucleases
cleave viral DNA sequences without methylation (bacterial DNA is methylated)
Tropism determined by
receptors on host cell
Internalized virions undergo
uncoating
Uncoating
genome is released from capsid
Assembly of new animal virions occurs in:
cytoplasm or nucleus
Envelope proteins (for enveloped viruses) are inserted into:
plasma membrane or organelle membrane
Release of animal virions from host via:
lysis or budding
animal DNA viruses use nucleus for:
genome replication and viral assembly.
animal DNA viruses use outside of nucleus for:
viral protein synthesis
animal RNA viruses:
only use outside of the nucleus
Papillomavirus
ds DNA; viral genome integration
Picornavirus
ss +RNA (rhinovirus, polio)
Retrovirus
enveloped; has 2 identical +RNA strands
Retrovirus infects:
T helper cells
Provirus
Retrovirus RNA → ds DNA integrated into host genome; may remain in latent state
Retrovirus exit
budding
Innate immunity
interferon (IF) produced by virus-infected cells
Interferon
induces production of anti-viral proteins in non-infected cells
Adaptive immunity
antibodies to virus (antigen interactions)
RNA interference
host expresses complimentary RNAs to viral genome to shut down expression