WBC identification Quiz

Myeloblast

Promeylocyte and Myelocyte

Metamyelocyte

What types of immature neutrophils should not be seen in circulating blood stream

Myeloblast, Promeylocyte, Myelocyte

What do you do if we see a myeloblast, promeylocyte or myelocyte in circulating blood stream?

Count them as separate cells in differential, and call to vet’s attention

band- neutrophil 

Segmented Neutrophil

How should you record band cells

Recorded as separate cells

Hyper-segmented Neutrophil 

what type of neutrophil is normal

Segmented Neutrophil

Toxic neutrophil, Dohle bodies 

Toxic neutrophil, Small basophilic granules

what is this and what are arrows pointing to

Toxic neutrophils

top arrow-cytoplasmic basophilia

left arrow- cytoplasmic vacuolation

right arrow- dohle bodies

Band neutrophil

Neutrophil

Toxic neutrophil

what disease is affecting these and what is it

Neutrophil, Histoplasma

what disease is affecting these and what is it

Neutrophil, Distemper

what disease is affecting these and what is it

Neutrophil, Ehrlichia or Anaplasma phagocytophilium

what disease is affecting these and what is it

Neutrophil, Ehrlichia or Anaplasma phagocytophilium

Atypical Lymphocytes

How to explain what a atypical lymphocyte looks like

Cytoplasm gets pulled out into ‘wonky” shapes when making slides

Reactive lymphocytes

what is it and what is it pointing to

atypical lymphocyte, Azurophilic granules (small reddish purple granules)

reactive lymphocyte

reactive lymphocyte

reactive lymphocyte

explan what a reactive lymphocyte looks like

larger

more cytoplasm

bluer cytoplasm

lacier chromatin

vacuoles

abnormal monocytes

abnormal monocytes

why do we get abnormal monocytes

EDTA tends to distort monocytes see irregular margins and more vacuoles

when do we see Pyknotic neutrophils and monocytes

early stage of apoptosis- programmed cell death

Pyknotic neutrophil 

Pyknotic neutrophil 

Pyknotic Monocyte

Plasma, Buffy coat, RBC’s

What is PCV

proportion (%) of blood composed of red blood cells

PCV potential errors

not mixing sample before filling tube

including the buffy coat in PCV value

Too much anticoagulant dilutes blood

centrifuge error- not enough rpms

plasma is made up of how much proteins (%)

5-10%

what proteins are in plasma

Albumin

globulins

fibrinogen

Potential errors in TP value

Glass shards-use unbroken end of tube

plasma cloudiness-hemolysis or lipemia

refractometer scratches or being dirty

what tube do we use for blood smears

purple top EDTA

lipemic sample

yellow one

icteric sample

left one

hemolyzed sample

name zones from left to right

body, monolayer, feathered edge

what do cells look like in body

cells piled on top of each other

not appropriate for differentials

what do cells look like in monolayer

cells are laying in single later close but not touching

appropriate area for differentials and estimates 

what does feathered edge look like

cells are very far apart with large gaps

not appropriate for differentials 

order of stains used for staining slides

methyl alcohol- fixer

Eosinate stain- reds

Methylene blue stain- purples

how many dips in each stain for staining slides

10

what is average total blood volume in all animals

8%

what is a safe amount of total blood volume we can remove from a healthy adult. why would we do this

20%, for blood transfusion donation

how much total blood volume is in each animals body weight.

hot blooded horses

cats,ruminants, lab animals, cold blooded horses

dogs

pigs

10-11% Body Weight – Hot-blooded horses
▪ 6-7% Body Weight - cats, ruminants, lab animals, cold-
blooded horses
▪ 8-9% Body Weight -dogs
▪ 5-6% Body Weight - pigs

what to do with microscope setting for differntials

light?

WBC’s estimate

platelets estimate 

condenser up and light source bright

40x for WBC objective lens

100x for platelet objective lens

how to calculate WBC estimate

count WBC in 10 feilds (40x)

divide by 10

x by 2,000

how to calculate platelet estimate

count all in 10 fields (100x)

divide by 10

x 20,000

how to get absolute WBCs

total WBC estimate X % of each WBC found on differential

ex: 40 neutrophils (0.4) X WBC estimate

what is a WBC absolute most useful for

evaluating health/disease

what is used on 100x

everything but WBC estimate

how much bigger are neutrophils than RBC

2-2.5 bigger than RBC

what is that dot at the bottom what is it found in

barr body, females (neutrophils)

why may neutrophils show up pink to red

too many dips in solution

what to look for in neutrophils

small granules

segmented nucleus

occasional band

what spceies

neutrophil-canine

what species

neutrophil, cat

what species

neutrophil, equine

what species

Neutrophil, bovine

how much bigger are lymphocytes then RBC’s in dogs and cats

1-1.5

how much bigger are lymphocytes then RBC’s in livestock

2-2.5

what is it, what is arrow pointing at

lymphocyte, scant cytoplasm

lymphocyte

lymphocyte

what animal is this in and what is it

dog,cat lymphocyte

what animal is this in and what is it

equine, bovine lymphocyte

what do monocytes look like

nucleus often seems frothy with dark spots and irregular shapes

big

often have vacuoles

small granules

how much bigger is a monocyte than a RBC

4-4.5

monocyte

monocyte

what animal is this in and what is it

canine, feline monocyte

what animal is this in and what is it

equine, bovine monocyte

Eosinophil

what does a eosinophil look like

large pink granules

segmented nucleus

a big larger than neautrophil

what animal is this in and what is it

canine, feline eosinophil

what animal is this in and what is it

Equine, bovine Eosinophil

what is this

mast cells- from cutaneous tumor in dog

what does a basophil look like

Dark blue granules

segmented nucleus

a bit larger than neutrophil 

what animal is this in and what is it

canine, feline basophil

what animal is this in and what is it

equine, bovine basophil

basophil

what is this called

platelet clump

purple things

platelets

right to left

RBC, platelet, WBC

what is the bottom purple thing

giant plateletes

Heterophil

heterophil

what species

heterophil, rabbit

what species 

heterophil, reptiles and birds

heterophil