monoclonal antibodies

monoclonal antibodies

antibody produced from genetically identical plasma cells

use of monoclonal antibodies in treatment

has a specific tertiary structure complementary to receptor

therapeutic drug attached to antibody

antibody binds to cell delivering drug directly to cell

use of monoclonal antibodies in medical diagnosis

has a specific tertiary structure

complementary to specific receptor

dye attached to antibody

antibody binds to receptor forming antigen antibody complex

ELISA test

specific antigens attached to bottom of well

potential antibodies added and washed to remove unbound antbodies

monoclonal antibody complementary to antibodies added with an enzyme attached

washed

colourless substrate added which causes colour change if enzyme present

purpose of a control well in an Elisa test

ensures enzyme causes the colour change

ensures all antibody has been washed away