Post-Lab Review 1: Labs 2-4

Flashcards covering key concepts from the post-lab review.

y in the standard curve equation

The absorbance value for that sample

x in the standard curve equation

The concentration of an unknown sample

Bradford assay

An assay that detects proteins in a solution

Reason for protein content difference in homogenates from liver and heart

Different function of two tissues account for variability in protein content and concentrations

Homogenates with high protein concentration

Darker bands on an SDS-PAGE gel

Amylocosidase

Breaks the glycosidic bonds within the polymer

Glucose oxidase

Will reduce water to become hydrogen peroxide

4-AAP and phenol

Glucose oxidase releases peroxide and peroxidase reduces it to red quinoneimine

True or False: The Y-axis label on your glycogen graph should be: "Glucose Levels (mg/ml)"

False. Glycogen graph should be Glucose concentration (mg/ml)

Functions indicated by calculating free glucose and glucose from glycogen

More glycogen indicates that the tissue is used by the body to store glucose until needed and More free glucose indicates that the tissue uses glycolysis to generate ATP

Concentration of solutes inside the cell relative to the concentration of solutes in solution containing 200 mM NaCl

The inside of the cell has lower concentration of solute compared to 200mM NaCl leading to net movement of water of the cell causing it to shrink

If the isotonic concentration of NaCl is 120 mM, what would the isotonic concentration of MgCl2 be?

NaCl dissociates into 2 particles (Na⁺ and Cl⁻); MgCl₂ dissociates into 3 particles (Mg²⁺ and 2 Cl⁻). MgCl2 concentration= 240/3 = 80mM

Controls of NaCl and Sucrose experiments

No solute- distilled water- negative control. Isotonic solute, and hypertonic solute- positive control