Glycolysis Worksheet/Chpt 17

Substrate level phosphorylation

a reaction in which the source of phosphorus is inorganic phosphate ion, not ATP

How many times does the dividend phase (5-10) occur per glucose molecule?

twice

glucose

Glucose-6-phosphate

Fructose-6-phosphate

Fructose 1, 6 bisphosphate

Dihydroxyacetone Phosphate

Glyceraldehyde 3- phosphate

1,3 bisphosphoglycerase

3-phosphoglycerate

2-phosphoglycerate

phosphoenolpyruvate

2-Phosphoglycerate w/o the water

pyruvate

What process is happening here?

phosphorylation of glucose

hexokinase

responsible for the phosphorylation of glucose

Isomerization of Glucose-6-Phosphate (G6P)

What process is happening here?

PhosphoGlucoIsomerase

responsible for the Isomerization of Glucose-6-Phosphate (G6P)

Phosphorylation of Fructose-6-phosphate (F6P)

What process is happening here?

Phosphofructo Kinase

responsible for phosphorylation of Fructose-6-phosphate (F6P)

cleavage of a carbon-carbon bond of F6P

what reaction is happening here?

Aldolase

responsible for cleavage of a carbon-carbon bond of F6P

isomerization dihydroxyacetone phosphate

what reaction is happening here?

Triphosphoisomerase

responsible for isomerization dihydroxyacetone phosphate

Oxidation and phosphorylation of glyceraldehyde-3-phosphate

what reaction is happening here?

glyceraldehyde-3-phosphate dehydrogenase

responsible for oxidation and phosphorylation of glyceraldehyde-3-phosphate

substrate level phosphorylation of 1,3-bisphosphoglycerase(BPG)

what reaction is happening here?

phosphoaldehyde kinase

responsible of substrate level phosphorylation of 1,3-bisphosphoglycerase(BPG)

isomerization of 3-phosphoglycerate (3PG)

what reaction is happening here?

phosphoglycerate mutase

responsible for isomerization of 3-phosphoglycerate (3PG)

dehydration of 2-phosphoglycerate (2PG)

what reaction is happening here?

Enolase

responsible for dehydration of 2-phosphoglycerate (2PG)

substrate level of phosphorylation of phosphoenolpyruvate

what reaction is happening here?

pyruvate kinase

responsible for substrate level of phosphorylation of phosphoenolpyruvate

1 & 3

What step(s) are ATP (and Mg) used for

6

What step(s) use NAD and phosphate

7 & 9

What step(s) produce ATP

1,3,9

Which steps are control points

Hexokinase, inhibited by glucose-6-phosphate

What enzyme occurs at step 1 and what is it inhibited by

Phosphofructo kinase, inhibited by ATP

What enzyme occurs at step 3 and what is it inhibited by

Fructose 2, 6 biphosphate

What enzyme occurs at step 3 and what is it activated by

pyruvate kinase, inhibited by ATP, Alanine, Acetyl CoA

What enzyme occurs at step 9 and what is it inhibited by

pyruvate kinase, Fructose 1, 6 bisphosphate

What enzyme occurs at step 9 and what is it activated by

which enzyme is involved in the committed step?

phosphofructo kinase

What molecule is required for step 6 (glyceraldehyde-3-phosphate dehydrogenase)

NAD⁺

1,3 biphosphoglycerate(after step 6) and phosphoenolpyruvate(after step 9)

High energy metabolites

Lactate fermentation

anaerobic metabolism of pyruvate

lactate fermentation function

recycling of NADH to NAD⁺

dead end

lactate can only revert back into pyruvate, which makes it a what

32 ATP

Net yield of ATP from ONE GLUCOSE