Gene Cloning Practice

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8 Terms

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Step 1

A plasmid is located in E. coli bacterium

2
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Step 2

The cell with DNA containing the gene of interest is isolated

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Step 3

Plasmid is cut with an enzyme

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Step 4

Cell's DNA is cut with the same enzyme

5
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Step 5

The targeted fragment and plasmid DNA are combined

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Step 6

DNA ligase joins the two DNA molecules together making the plasmid a complete RING again

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Step 7

The recombinant plasmid is taken up by bacterium through transformation

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Step 8

Bacterium reproduces