Gene Cloning Practice
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Last updated 6:16 AM on 12/8/25
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8 Terms
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Step 1
A plasmid is located in E. coli bacterium
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Step 2
The cell with DNA containing the gene of interest is isolated
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Step 3
Plasmid is cut with an enzyme
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Step 4
Cell's DNA is cut with the same enzyme
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Step 5
The targeted fragment and plasmid DNA are combined
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Step 6
DNA ligase joins the two DNA molecules together making the plasmid a complete RING again
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Step 7
The recombinant plasmid is taken up by bacterium through transformation
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Step 8
Bacterium reproduces
Note 
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